Phospho-MKK3 (Ser189) Rabbit Polyclonal Antibody#abs130952

abs130952-50ug

1-2 Weeks

201

abs130952-100ug

1-2 Weeks

301

Description

This gene encodes a protein kinase that exhibits dual specificity and is a member of the MAP kinase kinase family. It plays a crucial role in the signaling cascade mediated by MAP kinases, being activated by both mitogenic signals and environmental stress. Its main function involves phosphorylating and activating MAPK14/p38-MAPK. Furthermore, this kinase can be stimulated by insulin, making it essential for the expression of glucose transporter.

Other names

Mitogen-activated protein kinase kinase 3, also known as dual specificity mitogen-activated protein kinase kinase 3 or MAP kinase kinase 3, plays a significant role in cellular signaling pathways. It is commonly referred to as map2k3 or MAPKK3 and is involved in the activation of MAP kinases.
MEK3, another name for MAPK ERK kinase 3, functions as a protein kinase that phosphorylates MAPKs, leading to their activation. Additionally, it is referred to as mMKK3b or MP2K3_HUMAN, highlighting the various genetic variations and species-specific nomenclatures.
PRKMK3 or protein kinase, mitogen-activated, kinase 3, is another name for this protein enzyme. It acts as a crucial mediator in the intracellular response to stress and other external stimuli. Moreover, it is also known as SAPK kinase 2 or SAPKK2, indicating its involvement in stress-activated protein kinase pathways.
In summary, the dual specificity mitogen-activated protein kinase kinase 3 is a vital component in multiple signaling cascades. Its diverse aliases reflect the intricate nature of its functions and its relevance in various biological contexts.

Source

Rabbit

Specificity

The antibody known as Phospho-MKK3 (Ser189) is capable of detecting endogenous levels of MKK3, but only when it is phosphorylated at the serine 189 site. In other words, the antibody has specificity for recognizing the specific phosphorylated form of MKK3 at this particular site. This information is important for researchers who are interested in studying the phosphorylation status of MKK3 and its role in various cellular processes. By using this antibody, researchers can gain insights into the signaling pathways that involve MKK3 and identify potential therapeutic targets for various diseases.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. These dilutions should be used to achieve optimal results in the respective assays.

Mimicking the phosphorylation site of Serine 189, I propose the synthesis of a novel peptide derived from the human MKK3. The synthetic peptide will closely resemble the original sequence, preserving its critical information.

Properties

1mg/ml

Purification

Through the use of sequential chromatography on both phospho- and non-phospho-peptide affinity columns, a highly purified antibody has been isolated from rabbit serum via an affinity purification process. This antibody is the result of a rigorous process designed to achieve high affinity and specificity, making it an excellent tool for biological research and analysis.

Clonality

Polyclonal Antibody

Please store at a temperature of -20 °C for a duration of one year, and be sure to prevent any instances of freezing and thawing cycles. It is crucial to avoid subjecting the product to repetitive temperature fluctuations.

Storage buffer

This is a description of Rabbit IgG stored in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is stable at -20 °C and can be kept for up to 12 months from the date of receipt.

Target

Background

 Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Tissue specificity

Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Celluar localization

Cytosol;Nucleus;

P46734