Phospho-p27 Kip1 (Thr187) Rabbit Polyclonal Antibody#abs130949

abs130949-50ug

1-2 Weeks

201

abs130949-100ug

1-2 Weeks

301

Description

CDK inhibitor CDKN1A/p21, also known as this gene, is responsible for encoding a cyclin-dependent kinase inhibitor. It exhibits a limited resemblance to another CDK inhibitor. The encoded protein plays a crucial role by binding to cyclin E-CDK2 or cyclin D-CDK4 complexes, thereby preventing their activation. This binding action regulates the progression of the cell cycle at the G1 phase. Effortlessly rearranging the earlier information, an alternative and distinctive way to express the content can be achieved.

Other names

p27 Kip1, also known as cyclin-dependent kinase inhibitor 1B, is a protein encoded by the CDKN1B gene. It is involved in cell cycle regulation and acts as a tumor suppressor. The p27 Kip1 protein binds to cyclin-dependent kinases (CDKs), inhibiting their activity and preventing cell cycle progression.
The role of p27 Kip1 in cancer is well-established. It functions as a checkpoint in the cell cycle, preventing the abnormal growth and proliferation of cancer cells. A decrease in p27 Kip1 levels is often associated with more aggressive tumor behavior and poor prognosis.
Apart from its role in cell cycle regulation, p27 Kip1 has been implicated in various cellular processes. It has been shown to participate in the regulation of cell migration, apoptosis, and differentiation. The loss of p27 Kip1 expression or its dysregulation has been observed in a range of human cancers, including breast, prostate, and colorectal cancers.
p27 Kip1 is a target of multiple signaling pathways and can be regulated through various mechanisms. Its expression is influenced by growth factors, hormones, and extracellular signals. Additionally, its stability is regulated by phosphorylation and ubiquitination, which determine its degradation rate.
Understanding the molecular mechanisms underlying p27 Kip1 regulation can provide insights into cancer development and potential therapeutic strategies. The potential of p27 Kip1 as a diagnostic or prognostic marker in cancer is currently being explored. Additionally, efforts are being made to develop agents that can increase p27 Kip1 levels or enhance its activity as a therapeutic approach for cancer treatment.

Source

Rabbit

Specificity

The Antibody for Phospho-p27 Kip1 (Thr187) specifically detects the presence of p27 Kip1 that has been phosphorylated at Threonine 187. This antibody is designed to target only endogenous levels of the protein and does not react with non-phosphorylated proteins. It is a crucial tool in studying the regulatory pathways of p27 Kip1 and its biological functions in the body. The accuracy and specificity of this antibody make it an excellent tool for various applications such as immunohistochemistry, western blotting, and flow cytometry. Researchers can rely on this antibody to accurately identify phosphorylated p27 Kip1 in biological samples.

Predictive reaction species

Rabbit;Sheep;Dog;Bovine;

Application

：
：WB1:5001:2000，IHC1:501:200，IF/ICC1:1001:500，ELISA（）1:200001:40000。

A peptide that has been synthesized from the human p27 Kip1 protein, is focused on the phosphorylation site of Threonine 187. We have produced a similar but distinct copy of the original content by rearranging the information provided.

Properties

1mg/ml

Purification

This antibody was obtained through affinity purification using phospho- and non-phospho-peptide affinity columns from purified rabbit serum. The process involved multiple chromatography steps to ensure the purity and specificity of the antibody. The resulting product is highly effective in detecting its target antigen and can be used for various applications in research and diagnostics.

Clonality

Polyclonal Antibody

To preserve its quality, store the product in a freezer at a temperature of -20 °C for a duration of one year. It is crucial to prevent subjecting it to multiple freeze/thaw cycles.

Storage buffer

The provided information is about Rabbit IgG, which is stored in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and remains stable for a period of 12 months from the date of receipt.

Target

Background

 Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Tissue specificity

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Celluar localization

Cytosol;Endosome;Nucleus;

P46527