Phospho-CrkII (Tyr221) Rabbit Polyclonal Antibody#abs130948

abs130948-50ug

1-2 Weeks

201

abs130948-100ug

1-2 Weeks

301

Description

Crk is a protein that acts as an adaptor and has a unique SH2-SH3-SH3 domain structure. It is responsible for recruiting cytoplasmic proteins by interacting through the SH2-phospho-tyrosine mechanism. Crk can be phosphorylated by important proteins such as Abl, IGF-IR, and EGFR to trigger different cellular responses.

Other names

Crk adapter molecule; Avian sarcoma virus CT10 oncogene homolog (v-crk); CRK gene; CRK protein isoform 2; CRK protein isoform II; CRK_HUMAN protein; CRKII protein; FLJ38130 gene; OTTHUMP00000115366 gene; OTTHUMP00000198330 gene; p38 protein; Proto-oncogene C-crk; Proto-oncogene C-crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog; avian sarcoma virus CT10 oncogene homolog.

Source

Rabbit

Specificity

The Phospho-CrkII (Tyr221) Antibody specifically recognizes the phosphorylated form of CrkII at Tyrosine 221. It is designed to detect the endogenous levels of CrkII only when it is phosphorylated at this specific site. By targeting this phosphorylation event, this antibody provides valuable insights into the cellular signaling pathways involving CrkII and its associated functions. Its specificity and sensitivity make it a powerful tool for researchers studying the regulatory mechanisms and functional implications of CrkII phosphorylation.

Predictive reaction species

Xenopus;Chicken;Pig;Dog;Sheep;Bovine;Horse

Application

According to the original information, the recommended dilutions for Western Blot (WB) range from 1:500 to 1:2000. For Immunofluorescence (IF) and Immunocytochemistry (ICC), the recommended dilutions are between 1:100 and 1:500. In the case of ELISA using a peptide, the suggested dilutions are 1:20000 to 1:40000. It is important to acknowledge that the generated content may differ significantly from the original text, as it will be based on the language model's own understanding and generation capabilities.

A synthetic peptide has been created using human CrkII with a focus on the phosphorylation site of Tyrosine 221. Would it be possible to produce additional content based on this information?

Properties

1mg/ml

Purification

The antibody was obtained by utilizing purified rabbit serum and subjected to sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This enabled a highly specific purification process that led to the isolation of the desired antibody. The resulting product is highly effective in its intended use and is a testament to the intricate and meticulous purification process employed.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of minus 20 degrees Celsius for a period of one year. It is important to avoid exposing it to repeated cycles of freezing and thawing, as this could affect its effectiveness. Therefore, it is crucial to take caution when handling and storing the product to ensure that it retains its potency.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline, with a pH value of 7.4, 150 millimolar concentration of sodium chloride, and 0.02% sodium azide. It also includes 50% glycerol for preservation when stored at -20 °C. This solution is stable for up to 12 months from the date of receipt. Please follow the storage instructions to ensure product longevity.

Target

Background

Crk-II isoform is responsible for regulating various cellular processes including cell adhesion, spreading, and migration. It plays a crucial role in activating MAPK8 upon attachment, leading to membrane ruffling and cell motility. These actions are dependent on the activation of the Rac protein. Additionally, it facilitates the phagocytosis of apoptotic cells and enhances cell motility by interacting with proteins DOCK1 and DOCK4. Another significant function of Crk-II is its potential regulation of the EFNA5-EPHA3 signaling pathway.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

P46108