Phospho-ICAM-1 (Tyr512) Rabbit Polyclonal Antibody#abs130716

abs130602-50ug

1-2 Weeks

201

abs130602-100ug

1-2 Weeks

301

Description

ICAM1 is a protein that belongs to the immunoglobulin superfamily and resides on the surface of cells. Specifically, it functions as a receptor for the LFA-1 protein, which is important for the adhesion and migration of leukocytes. Additionally, ICAM1 is a receptor for the Rhinovirus, a common cause of the common cold. This protein is most commonly found on immune cells and endothelial cells.

Other names

Several names have been associated with the monoclonal antibody BB2, including BB 2, CD 54, and CD54. This antibody identifies a cell surface glycoprotein known as P3.58, which also goes by the name ICAM 1, ICAM-1, or ICAM1. This protein is responsible for playing a critical role in the cellular adhesion process, and it serves as a receptor for human rhinovirus, making it a target for antiviral therapies. Other aliases for ICAM 1 include MyD 10, MyD10, MALA 2, and MALA2, and it is also referred to as the surface antigen of activated B cells, BB2. Overall, this protein is an essential component of the immune response and is involved in allowing immune cell migration to sites of inflammation.

Source

Rabbit

Specificity

The Phospho-ICAM-1 (Tyr512) Antibody is capable of detecting the levels of endogenous ICAM-1, but only when it is phosphorylated at Tyrosine 512. This antibody specifically recognizes the phosphorylated form of ICAM-1 and can assist in the identification of certain cellular signaling pathways that involve this particular modification. By identifying the phosphorylation status of ICAM-1, researchers can gain insights into the regulation of cellular communication and immune responses.

Application

The recommended dilution range for this antibody is WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000. It is important to note that these dilutions are based on the original text information and should be carefully followed to obtain optimal results. By rearranging the content and emphasizing its significance, we hope to ensure accurate and effective usage of this antibody for various research applications.

A peptide synthesized from the human ICAM-1 molecule, specifically around the Tyrosine 512 phosphorylation site, has been produced. The sequence of the peptide closely resembles the original human ICAM-1 molecule, with a focus on the specific phosphorylation site. The purpose of this synthesized peptide is to potentially aid in research regarding ICAM-1 and its functions, as well as potential therapeutic uses in the future.

Properties

1mg/ml

Purification

The process used to obtain the antibody involved purification of rabbit serum through sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This resulted in a highly purified antibody that is similar in composition to the original serum.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is important to store it at a temperature of -20 °C for a duration of one year. It is advised to avoid subjecting the product to repetitive freeze/thaw cycles. Taking these precautions will ensure the optimal preservation of the product over an extended period of time.

Storage buffer

The Rabbit IgG is suspended in a solution of phosphate buffered saline with a pH of 7.4, 150mM of NaCl, 0.02% of sodium azide and 50% glycerol. For optimal preservation, store the product at a temperature of -20 °C. Its shelf life is guaranteed to be 12 months from the date of receipt, making it an ideal product for long-term use. To ensure consistent quality, we recommend following all storage instructions provided.

Target

Background

The interaction between ICAM proteins and the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) plays a crucial role in leukocyte trans-endothelial migration. Specifically, when ICAM1 engages with LFA-1, it triggers the activation of ARHGEF26/SGEF and RHOG, leading to the formation of endothelial apical cups. These cups are essential for facilitating the migration of leukocytes across the endothelium. Hence, the engagement of ICAM1 with LFA-1 initiates a cascade of events that promote the assembly of these specialized structures and enable efficient leukocyte trans-endothelial migration.

Celluar localization

Extracellular region or secreted;Plasma Membrane;