Phospho-c-PLA2 (Ser505) Rabbit Polyclonal Antibody#abs130954

abs130954-50ug

1-2 Weeks

201

abs130954-100ug

1-2 Weeks

301

Description

cPLA2 is an enzyme that requires calcium ions as a cofactor and functions as a phospholipase A2. Its primary role is to break down phospholipids in cell membranes and release arachidonic acid. Specifically, it targets arachidonyl phospholipids in the sn-2 position, selectively hydrolyzing them and liberating arachidonic acid. This process is crucial for the production of various bioactive lipid mediators, including prostaglandins, leukotrienes, and thromboxanes, that play important roles in inflammation and other physiological processes. By studying cPLA2 and its functions, researchers have gained valuable insight into the complex mechanisms underlying lipid metabolism and signaling in cells.

Other names

Cytosolic phospholipase A2 (cPLA2), also known as calcium-dependent phospholipid binding protein, is an enzyme that plays a crucial role in cellular processes. It belongs to the phospholipase A2 group IVA family. Some other names associated with cPLA2 include cPLA2 alpha, lysophospholipase, MGC126350, PA24A_HUMAN, phosphatidylcholine 2 acylhydrolase, phospholipase A2 group 4 A, and phospholipase A2 group IVA (cytosolic calcium dependent). Its primary function is to catalyze the hydrolysis of phosphatidylcholine, leading to the liberation of free fatty acids and lysophosphatidylcholine. This enzymatic activity is dependent on the presence of calcium ions. cPLA2 plays a significant role in various cellular processes, including cell signaling, inflammation, lipid metabolism, and membrane remodeling. Understanding the intricate workings of cPLA2 provides valuable insights into different pathological conditions and potential therapeutic targets.

Source

Rabbit

Specificity

The Phospho-c-PLA2 (Ser505) Antibody is designed to specifically recognize the phosphorylated form of c-PLA2 at Serine 505. It is capable of detecting endogenous levels of c-PLA2 only when this protein is modified through phosphorylation at the specified site. This antibody is an essential tool for researchers studying signal transduction pathways involving c-PLA2 and its role in various cellular processes. By detecting this phosphorylation event, Phospho-c-PLA2 (Ser505) Antibody enables a deeper understanding of the complex regulatory mechanisms underlying c-PLA2 activity and its impact on cellular function.

Application

ELISA (peptide) 1:20000-1:40000, WB 1:500-1:2000, IHC 1:50-1:200, and IF/ICC 1:100-1:500 are the recommended dilutions for the various techniques. These dilutions represent the optimal ranges for achieving accurate and reliable results. It is essential to follow the suggested dilutions as they have been determined through experimental validation. By using the appropriate dilutions, one can ensure the successful application of these techniques in immunological assays.

Serine 505 is the phosphorylation site of human c-PLA2, and a synthesized peptide has been developed based on this region. The peptide is derived from human c-PLA2 and is designed to closely resemble the amino acid sequence around the phosphorylation site.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing in order to maintain its stability and effectiveness. Therefore, proper storage is essential to maintain the integrity of the product and ensure its long-term viability.

Storage buffer

This product consists of Rabbit IgG suspended in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20°C and is stable for up to 12 months from the date of receipt.

Target

Background

This enzyme is capable of hydrolyzing arachidonyl phospholipids at the sn-2 position to release arachidonic acid. Its lysophospholipid activity adds to its role in triggering the inflammatory response. As a result, it is strongly implicated in the initiation of inflammation.

Tissue specificity

Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.

Celluar localization

Cytosol;Endoplasmic reticulum;Golgi apparatus;Mitochondrion;

P47712