Phospho-SOX-9 (Ser181) Rabbit Polyclonal Antibody#abs130955

abs130955-50ug

1-2 Weeks

201

abs130955-100ug

1-2 Weeks

301

Description

SOX9 is a crucial factor in the proper development of the skeletal system. Its role as a transcription factor for several genes involved in chondrogenesis enables it to regulate their expression. CMD1, or campomelic dysplasia, is often caused by defects in SOX9. Its significance lies in its ability to control the expression of other genes in the process of skeletal growth and development.

Other names

Autosomal sex reversal in campomelic dysplasia, also known as CMD 1 or CMPD 1, is a genetic disorder caused by mutations in the SOX 9 gene. This gene encodes for the SRY (sex determining region Y) box 9 protein, which plays a crucial role in the development of male sexual characteristics and skeletal formation. Mutations in SOX 9 can result in individuals with female genitalia but with male chromosomes (SRXX2) or individuals with male genitalia but with female chromosomes (SRXY10). Campomelic dysplasia autosomal, characterized by skeletal abnormalities and other congenital defects, is frequently associated with mutations in the SOX 9 gene. The transcription factor SOX-9, encoded by the SOX 9 gene, regulates the expression of various genes involved in skeletal development. Understanding the underlying molecular mechanisms of SOX 9 is essential in unraveling the pathogenesis of campomelic dysplasia and related disorders.

Source

Rabbit

Specificity

The Phospho-SOX-9 (Ser181) Antibody specifically recognizes the presence of phosphorylated SOX-9 at Serine 181 in the body's natural state. By only detecting this phosphorylated form, it allows for a more precise analysis of SOX-9 levels. Let me know if you need any further clarification or information.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse

Application

To properly use this antibody, recommended dilutions are as follows: for western blotting, dilute at 1:500 to 1:2000; for immunohistochemistry, dilute at 1:50 to 1:200; for immunofluorescence and immunocytochemistry, dilute at 1:100 to 1:500. For ELISA using the peptide, the recommended dilution is 1:20000 to 1:40000. Please ensure proper dilution for optimal results.

A highly similar content can be generated by rearranging the original statement as follows:
The phosphorylation site of Serine 181 in human SOX-9 serves as the basis for synthesizing a peptide. This synthesized peptide is derived from the aforementioned protein and shares a high degree of similarity to it.

Properties

1mg/ml

Purification

The highly purified antibody was obtained from rabbit serum using a sophisticated technique called affinity purification, which entailed sequential chromatography on columns containing phospho- and non-phospho-peptide affinity. The resulting antibody is an excellent candidate for use in various biomedical research applications, owing to its high purity and specificity. This methodology allows for the production of high-quality antibodies that have a wide range of applications, particularly in the field of medical research.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is important to store it at -20 °C for a duration of one year. It is crucial to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

Phosphate buffered saline with Rabbit IgG is a solution with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and remains stable for 12 months from the date of receipt.

Target

Background

 69kDa

Celluar localization

P48436