Phospho-C/EBP- Alpha (Ser21) Rabbit Polyclonal Antibody#abs130958

abs130958-50ug

1-2 Weeks

201

abs130958-100ug

1-2 Weeks

301

Description

This intronless gene encodes a bZIP transcription factor, which plays a vital role in gene regulation. It exhibits the ability to bind to specific promoters and enhancers either as a homodimer or by forming heterodimers with CEBP-beta and CEBP-gamma, proteins that are closely related. Research has demonstrated that this encoded protein can interact with the promoter region and effectively control the expression of the leptin gene. Leptin is a crucial protein involved in maintaining body weight homeostasis. By modulating its expression, the encoded protein contributes to the regulation of body weight.

Other names

Here are some variations of the text:
1. This list includes several proteins and enzymes that play important roles in apoptosis, also known as programmed cell death. Among them are the apoptotic cysteine protease, which regulates the breakdown of cellular components during apoptosis, and the caspase 8 precursor, which is a crucial initiator of the apoptotic cascade. Other names for these proteins include ICE-like apoptotic protease 5, FLICE, and MCH5.
2. The CCAAT/enhancer binding protein alpha, or C/EBP alpha, is a transcription factor that regulates gene expression, including in the context of adipocyte differentiation and liver metabolism. Other names for C/EBP alpha include CAP4 and CBF-A.
3. One of the key proteins involved in apoptosis is FADD homologous ICE/CED 3 like protease, which is also known as MACH, ICE8, or FLICE. This enzyme plays a critical role in initiating the death receptor pathway of apoptosis. Another important protein in this pathway is MORT1 associated CED 3 homolog, which is involved in activating caspases.
4. Some of the proteins on this list have multiple names, highlighting the fact that identification and characterization of proteins can be a complex endeavor. However, by understanding the function and regulation of these proteins, researchers can gain insight into fundamental biological processes such as cell death, gene expression, and cellular differentiation.

Source

Rabbit

Specificity

The antibody Phospho-C/EBP-alpha (Ser21) is capable of detecting levels of endogenous C/EBP-alpha specifically when it is phosphorylated at Serine 21. To emphasize, the generated content should not follow the format and structure of the original text, rather, it should provide a completely different perspective using a language model.

Predictive reaction species

Pig;Bovine;

Application

The recommended dilution ranges for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA(peptide) are 1:20000-1:40000. These dilution ranges ensure optimal results and reliable data for each respective assay. It is important to follow these recommendations to achieve accurate and reproducible results in your experiments. Diluting the samples within these ranges will allow for the detection and quantification of your target of interest while minimizing background signal.

A peptide that has been synthesized from human C/EBP-alpha, specifically around the phosphorylation site of Serine 21, has been developed. The content generated from this information must be highly similar but with rearranged wording.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

One year storage is recommended at -20 °C. It is important to prevent repeated freezing and thawing cycles. Please ensure that the content generated is not in the format of a conversation but rather a completely different way of speaking using the language model.

Storage buffer

：IgG-20°C，，pH7.4，150mM，0.02%50%。12。

Target

Background

 Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity).

Celluar localization

Nucleus;

P49715