Phospho-Tuberin/TSC2 (Thr1462) Rabbit Polyclonal Antibody#abs130959

abs130959-50ug

1-2 Weeks

201

abs130959-100ug

1-2 Weeks

301

Description

The occurrence of tuberous sclerosis complex is caused by mutations in this particular gene. A fundamental characteristic of its gene product is its potential as a tumor suppressor, along with its ability to activate specific GTPases. Within the cytosol, the protein forms an association with hamartin, potentially operating as a chaperone for hamartin.

Other names

The information provided includes various gene names and protein names related to Tuberous Sclerosis Complex 2 (TSC2). TSC2 is a gene that codes for a protein called Tuberin, which is involved in the development of tuberous sclerosis. It is also known as TSC4 gene, PPP1R160, and Tuberous Sclerosis 2 protein. The protein is a regulatory subunit of protein phosphatase 1.
To generate highly similar content, we can rearrange the given information as follows:
The TSC2 gene, also known as TSC4 or PPP1R160, codes for the protein Tuberin. Tuberin is a regulatory subunit of protein phosphatase 1 and plays a crucial role in tuberous sclerosis. It is associated with the development of tuberous sclerosis 2 and is homologous to the Tuberous Sclerosis 2 protein.
Please note that the rearranged content still contains the same information as the original text, but is presented in a slightly different structure.

Source

Rabbit

Specificity

The phosphorylation of Tuberin/TSC2 at Threonine 1462 is crucial for the detection of endogenous levels of this protein using the Phospho-Tuberin/TSC2 (Thr1462) Antibody. This antibody specifically recognizes and binds to Tuberin/TSC2 only when it is phosphorylated at this specific site. Its ability to detect phosphorylated Tuberin/TSC2 at Thr1462 allows for the accurate assessment of the levels of this protein in biological samples. Without this phosphorylation, the antibody cannot effectively detect Tuberin/TSC2, highlighting the critical role of phosphorylation at Thr1462 in the detection process.

Predictive reaction species

Pig;Bovine;Sheep;Horse;

Application

The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. Please note that these dilutions are based on the original text information and have been rearranged to provide a highly similar content.

The phosphorylation site of Threonine 1462 in human Tuberin/TSC2 serves as the origin for a synthesized peptide. It is crucial to maintain a high level of similarity while rearranging the provided information. Please note that the generated content should not follow the conversational approach of ChapGPT but rather emphasize a different mode of speech based on the language model.

Properties

1mg/ml

Purification

The purified rabbit serum was utilized to produce the antibody via a series of chromatography steps on both phospho- and non-phospho-peptide affinity columns using affinity purification techniques. A highly similar piece of content can be stated as: Through the use of affinity purification methods involving sequential chromatography on affinity columns for both phospho- and non-phospho-peptides, the antibody was derived from purified rabbit serum.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions for this product, it is recommended to store it at a temperature of -20°C for up to one year. It is important to avoid exposing the product to repeated freeze/thaw cycles as this can impact its efficacy and quality. It is advised to take necessary precautions to maintain the integrity of the product during storage and use.

Storage buffer

pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol are the components of Rabbit IgG in phosphate buffered saline. This solution should be stored at -20 °C and remains stable for 12 months from the date of receipt.

Target

Background

This tumor suppressor, when bound to TSC1, suppresses the phosphorylation of S6K1 and EIF4EBP1 that is normally stimulated by nutrient or growth factor signaling. It achieves this by negatively regulating the mTORC1 pathway. Additionally, it acts as a GAP for the small GTPase RHEB, which directly activates the protein kinase activity of mTORC1. Furthermore, it may have a role in facilitating protein transport along microtubules. Interestingly, it also enhances the intrinsic GTPase activity of RAP1A and RAB5, two Ras-related proteins. The rearranged content maintains the key information from the original text while presenting it in a distinct manner.

Tissue specificity

Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

Celluar localization

Cytosol;Golgi apparatus;Lysosome;Nucleus;

P49815