Phospho-VASP (Ser157) Rabbit Polyclonal Antibody#abs130962

abs130962-50ug

1-2 Weeks

201

abs130962-100ug

1-2 Weeks

301

Description

Vasodilator-stimulated phosphoprotein (VASP) belongs to the Ena-VASP protein family, which comprises proteins that possess an EHV1 N-terminal domain. This unique domain enables them to bind to proteins that contain E/DFPPPPXD/E motifs and facilitates the localization of Ena-VASP proteins to focal adhesions.

Other names

Vasodilator-stimulated phosphoprotein (VASP), also known as Vasodilator stimulated phosphoprotein or VASP_HUMAN, is a protein involved in regulating vasodilation. It has been extensively studied due to its role in cell signaling and cytoskeletal rearrangement. VASP functions as a vasodilator by promoting the relaxation of blood vessels, thereby increasing blood flow. This protein is commonly found in endothelial cells, platelets, and smooth muscle cells. Its phosphorylation is regulated by various kinases, such as protein kinase A and C, which further modulate its activity. VASP has been implicated in multiple physiological processes, including blood pressure regulation, platelet aggregation, and wound healing. Its importance in cardiovascular health makes VASP a potential target for therapeutic interventions in various cardiovascular diseases. Overall, VASP plays a critical role in maintaining vascular homeostasis and ensuring proper blood flow throughout the body.

Source

Rabbit

Specificity

The Antibody for Phospho-VASP (Ser157) is sensitive to the presence of VASP only when it is phosphorylated at Serine 157. This means that it can specifically detect the level of endogenous VASP that is phosphorylated at this site. The antibody is designed to provide reliable and accurate results, and its specificity ensures that any changes in the phosphorylation state of VASP at Serine 157 can be accurately detected. Overall, the Phospho-VASP (Ser157) Antibody is a powerful tool for researchers who are investigating the role of VASP in various physiological and pathological processes.

Application

For Western blotting, a dilution of 1:500-1:2000 is recommended. In immunohistochemistry, the recommended dilution range is 1:50-1:200. For immunofluorescence and immunocytochemistry, a dilution range of 1:100-1:500 is recommended. If conducting an ELISA using a peptide, the recommended dilution range is 1:20000-1:40000. It is important to note that dilutions may need to be optimized for individual experiments and sample types.

A highly similar content can be generated by rearranging the above information about a synthesized peptide derived from human VASP around the phosphorylation site of Serine 157. Please note that the generated content will be based on the original text information. It is important to avoid generating content using the dialogue approach of ChapGPT and instead opt for generating text that is completely different.

Properties

1mg/ml

Purification

The affinity-purified antibody is derived from rabbit serum that has undergone sequential chromatography on phospho- and non-phospho-peptide affinity columns to ensure purification. Please note that the generated content should be distinctly different from the conversation generated by ChapGPT.

Clonality

Polyclonal Antibody

To maintain optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. However, it is important to avoid subjecting the product to repeated freeze/thaw cycles as this may impact its stability and effectiveness. So, it's crucial to handle the product with care and store it under appropriate conditions to ensure its quality is maintained for longer.

Storage buffer

This product contains rabbit IgG in a solution of phosphate buffered saline (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol. It should be stored at -20 °C and is stable for up to 12 months from the date of receipt. Please note that this information has been rearranged to provide a highly similar but unique description of the product.

Target

Background

VASP proteins are crucial in various cellular processes that rely on the restructuring of the cytoskeleton and the establishment of cell polarity. These processes encompass axon guidance, the dynamics of lamellipodia and filopodia, platelet activation, and cell migration. VASP functions as a promoter of actin filament elongation by safeguarding the growing end of actin filaments against capping and accelerating actin polymerization when capping protein is present. It achieves this by facilitating the transfer of actin monomers bound to profilin onto the growing end of actin filaments. Notably, VASP also contributes to the movement of Listeria monocytogenes within host cells, relying on actin-based mobility. Furthermore, VASP regulates actin dynamics in platelets and holds significance in the regulation of platelet aggregation.

Tissue specificity

Highly expressed in platelets.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Plasma Membrane;

P50552