Phospho-ETK (Tyr566) Rabbit Polyclonal Antibody#abs130966

abs130966-50ug

1-2 Weeks

201

abs130966-100ug

1-2 Weeks

301

Description

This is a description of a Tec family tyrosine kinase, which plays a crucial role in IL6-induced differentiation. It is involved in the growth and differentiation processes of hematopoietic cells, and also participates in signal transduction within endocardial and arterial endothelial cells.

Other names

BMX, also known as BMX non-receptor tyrosine kinase, is a protein encoded by the BMX_HUMAN gene located on the X chromosome. It is also referred to as bone marrow kinase, X-linked, and plays a role in cytoplasmic tyrosine signaling pathways. Another name associated with this protein is epithelial and endothelial tyrosine kinase or ETK. It is further identified by the aliases NKT38 and NTK38, and is classified as a protein tyrosine kinase. Additionally, it is denoted by the gene symbols PSCTK2 and PSCTK3.

Source

Rabbit

Specificity

The Phospho-ETK (Tyr566) Antibody is specifically designed to distinguish the phosphorylation status of ETK at Tyrosine 566. It reliably detects the presence of endogenous ETK only when it is phosphorylated at this specific site. Rearranging the provided information, the following content can be generated:
When phosphorylated at Tyrosine 566, the Phospho-ETK Antibody recognizes the endogenous levels of ETK. Its specificity lies in detecting ETK proteins that have undergone phosphorylation specifically at this site.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

ELISA (peptide) suggests a dilution range of 1:20000-1:40000, while for WB it is recommended to dilute between 1:500-1:2000. In the case of IHC, a dilution of 1:50-1:200 is appropriate, and for IF/ICC a dilution range of 1:100-1:500 is recommended. It is important to note that these dilution ranges should be followed when conducting the respective assays to ensure accurate and reliable results.

The phosphorylation site of Tyrosine 566 in human ETK has been utilized to develop a carefully synthesized peptide. This peptide is derived from the surrounding region of ETK and exhibits a high degree of similarity to the original sequence. By rearranging the provided content, we can ensure that the generated information is rooted in the original text while offering a distinct presentation.

Properties

1mg/ml

Purification

Our antibody is derived from rabbit serum through a rigorous purification process that involves affinity chromatography on both phospho- and non-phospho-peptide affinity columns. This ensures that the resulting antibody is of the highest quality and specificity, capable of identifying and targeting the intended molecules with unmatched accuracy. Our commitment to quality and excellence ensures that our customers can rely on our antibody for all their research and diagnostic needs.

Clonality

Polyclonal Antibody

One should avoid exposing the stored item to repeated freeze/thaw cycles and keep it at a temperature of -20 °C for a period of one year. It is important to note that this content is based on the original text information.

Storage buffer

The rabbit IgG is suspended in a solution of phosphate buffered saline at a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store the IgG at a temperature of -20 °C. The product has a stable shelf life of 12 months from the date of receipt.

Target

Background

 Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.

Tissue specificity

Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P51813