Phospho-STAT2 (Tyr690) Rabbit Polyclonal Antibody#abs130967

abs130944-50ug

1-2 Weeks

201

abs130944-100ug

1-2 Weeks

301

Description

The STAT protein family encompasses the protein encoded by this particular gene. These proteins are activated in response to cytokines and growth factors, with associated kinases phosphorylating them. Once phosphorylated, STAT proteins either form homo- or heterodimers which are then able to translocate to the cell nucleus. From here, they act as transcription activators, facilitating the regulation of gene expression.

Other names

The Homo sapiens interferon alpha induced transcriptional activator, also known as ISGF3 or Stat2, is a crucial signaling protein that is involved in the regulation of gene expression. It is induced by interferon alpha and plays a key role in the immune response, particularly in the antiviral defense mechanism. STAT2 exists as a 113kD protein and is a member of the signal transducer and activator of transcription protein family. It functions by forming a complex with other transcription factors, such as ISGF3, which binds to the promoter region of interferon-stimulated genes and activates their transcription. Additionally, STAT2 is also involved in mediating cytokine signaling and regulating cell growth and differentiation. Overall, the significance of STAT2 in immune system function has made it an important target for the development of novel therapeutics against infectious diseases and cancer.

Source

Rabbit

Specificity

This antibody, called Phospho-STAT2 (Tyr690) Antibody, recognizes STAT2 protein levels only when phosphorylated at Tyrosine 690, which is an endogenous state. This means that it can detect the specific modification of STAT2 at that particular site.

Application

The recommended dilution for using this antibody in WB is between 1:500 to 1:2000. For IHC, a dilution range of 1:50 to 1:200 is suggested. IF/ICC experiments should be performed using a dilution of 1:100 to 1:500. When using this peptide for ELISA, a dilution range of 1:20000 to 1:40000 would be appropriate. It is important to note that all dilutions should be optimized for each individual experiment to achieve the best results.

Around the phosphorylation site of Tyrosine 690, a synthesized peptide has been created from human STAT2. It is important to note that the following content does not adhere to the conversation style generated by ChapGPT, but rather provides a distinct way of discussing the information using a language model to generate text.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

To ensure long-term storage, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to any repeated freeze/thaw cycles, as this can potentially damage the integrity of the sample. Therefore, it is crucial to handle the product with care and follow the recommended guidelines to maintain its quality over time.

Storage buffer

The storage conditions for Rabbit IgG are as follows: The solution is in phosphate buffered saline with pH 7.4, 150mM NaCl, and 0.02% sodium azide. Additionally, the solution contains 50% glycerol, and should be stored at a temperature of -20°C. This solution is stable for up to 12 months from the date of receipt, providing a long shelf life for reliable use in experiments.

Target

Background

This protein, known as STAT1, is an important signal transducer and activator of transcription. It plays a critical role in transmitting signals from type I interferons, such as IFN-alpha and IFN-beta. When these interferons bind to their receptors on the cell surface, they activate two kinases called TYK2 and JAK1, which then phosphorylate STAT1 and STAT2 on tyrosine residues. The phosphorylated STAT proteins then dimerize and bind to another protein, IRF9, to form a transcription factor called ISGF3. This complex enters the nucleus and binds to specific DNA sequences, known as ISREs, to activate the transcription of genes that promote an antiviral state in the cell.
In addition to its role in interferon signaling, recent studies have shown that STAT1 also plays a role in regulating mitochondrial fission, a process by which cells divide their mitochondria into smaller fragments. Specifically, STAT1 modulates the activity of a protein called DNM1L, which is responsible for the fission process. By phosphorylating DNM1L at specific sites, STAT1 can activate or deactivate its GTPase activity, thereby regulating mitochondrial fission. These findings suggest that STAT1 may have broader roles in cellular physiology beyond its canonical function in interferon signaling.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P52630