Phospho-HER2 (Tyr1248) Rabbit Polyclonal Antibody#abs130697

abs1306097-50ug

1-2 Weeks

201

abs130697-100ug

1-2 Weeks

301

Description

The receptor tyrosine kinase family known as the epidermal growth factor (EGF) receptor includes a specific gene coding for a protein. However, this protein doesn't have its own domain for binding growth factors, thus making it unable to bind to these factors effectively.

Other names

ErbB2, also known as HER2 or NEU, is a viral oncogene homolog that is associated with several types of cancers, including neuroblastoma and glioblastoma. It is a receptor tyrosine kinase that plays a crucial role in cellular growth and proliferation. The protein is encoded by the ERBB2 gene and is also referred to as CD340 or MLN19. Its overexpression is often observed in metastatic lymph nodes and is implicated in the progression of these cancers. ErbB2 is a target for therapeutic intervention and is known to be inhibited by herstatin. The avian homolog of this oncogene, V erb b2, is also implicated in erythroblastic leukemia.

Source

Rabbit

Specificity

The Phospho-HER2 (Tyr1248) Antibody is specifically designed to detect the levels of HER2 that have been phosphorylated at Tyrosine 1248. It is capable of accurately identifying endogenous levels of this protein only under these conditions. The antibody is therefore a valuable tool for researchers studying HER2 signaling pathways and related cellular processes. By targeting this specific site, the Phospho-HER2 (Tyr1248) Antibody provides insights into the molecular mechanisms involved in HER2 activation and subsequent downstream effects. Its sensitivity and specificity make it an indispensable reagent for a wide range of applications in basic and clinical research.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig

Application

The dilution ratios for Western Blotting (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the suggested dilution ratios are 1:50 to 1:500. As for Immunofluorescence/Immunocytochemistry (IF/ICC), the appropriate dilution ratios are 1:100 to 1:500. Lastly, for Enzyme-linked Immunosorbent Assay (ELISA) using peptide antigens, the recommended dilution ratios are 1:20000 to 1:40000.

Tyrosine 1248 is an important phosphorylation site found in human HER2, and a synthesized peptide has been developed based on this site. This peptide is derived from the human HER2 protein and demonstrates a high similarity to the original sequence. Its composition and structure have been arranged to ensure maximum resemblance to the natural HER2 molecule. By focusing on Tyrosine 1248, this synthesized peptide aims to mimic the phosphorylation state and biological function associated with this specific site in HER2.

Properties

1mg/ml

Purification

The antibody is  from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Tissue specificity

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Celluar localization

Cytosol;Endosome;Nucleus;Plasma Membrane;

P04626