Phospho-Trk A (Tyr680+Tyr681) Rabbit Polyclonal Antibody#abs130700

abs130700-50ug

1-2 Weeks

201

abs130700-100ug

1-2 Weeks

301

Description

The genetic information within this gene encodes a vital member of the neurotrophic tyrosine kinase receptor (NTKR) family. Specifically, this gene produces a receptor that is embedded within the cell membrane. When neurotrophins bind to this receptor, it triggers a process called phosphorylation. This process involves the addition of phosphate groups to the receptor itself, as well as other members of the MAPK pathway. The activation of this kinase receptor leads to cell differentiation and may also play a crucial role in determining the specific subtypes of sensory neurons.
It is noteworthy that mutations occurring in this gene have been linked to several significant medical conditions. These conditions include congenital insensitivity to pain, anhidrosis (the inability to sweat), self-mutilating behavior, mental retardation, and even cancer. Therefore, any alterations or disruptions in this gene's functioning can have profound consequences on an individual's health.
Furthermore, scientists have identified alternative transcriptional splice variants of this gene. However, out of these variants, only three have been thoroughly studied and characterized to date. These variants likely possess unique functions and may contribute to the complexity and versatility of the overall genetic regulation mediated by this gene.

Other names

NTRK1, also known as Trk A or high affinity nerve growth factor receptor, is a tyrosine kinase receptor involved in neurotrophic signaling. It plays a crucial role in growth and development, particularly in the nervous system. The precursor form of this receptor, known as p140-TrkA, undergoes a transformation to p14-TrkA, which is associated with oncogenic activity. The slow nerve growth observed in the absence of Trk A highlights its importance in neuronal function. Overall, NTRK1 is a key player in nerve growth and function.

Source

Rabbit

Specificity

The Phospho-Trk A (Tyr680+Tyr681) Antibody can specifically detect Trk A when it is phosphorylated at Tyrosine 680 and 681 and at endogenous levels. This antibody is designed to recognize and bind to specific molecular regions on the Trk A protein, allowing for the detection of post-translational modifications. Through this targeted detection, scientists can gain insights into the regulation and function of Trk A in various biological systems. Therefore, this antibody is a valuable tool for researchers studying Trk A signaling pathways and related cellular processes.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Pig;Dog;Bovine;Sheep

Application

The recommended dilutions for this antibody are as follows: 1:500-1:2000 for western blotting, 1:50-1:500 for immunohistochemistry, and 1:20000-1:40000 for ELISA (using peptide). It's important to note that these dilutions may vary depending on the specific application and experimental conditions, so some optimization may be necessary. However, these dilutions should serve as a good starting point for most experiments. Overall, this antibody is a versatile tool for various immunodetection methods and can be used in a wide range of research applications.

A peptide has been synthesized from the human Trk A protein, specifically around the Tyrosine 680 and Tyrosine 681 phosphorylation site. The peptide shares high similarity to the original protein sequence and could potentially be used for further research or therapeutic purposes.

Properties

1mg/ml

Purification

The purified rabbit serum was utilized to produce the antibody through sequential chromatography on both phospho- and non-phospho-peptide affinity columns using affinity purification techniques. This process ensured that the resulting antibody is highly specific and has a high degree of similarity to the original serum.

Clonality

Polyclonal Antibody

To preserve its quality, it is recommended to store the item in a freezer at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the item to repeated freeze/thaw cycles.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1850821, PubMed:1849459, PubMed:1281417, PubMed:8325889, PubMed:15488758, PubMed:17196528). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.

Tissue specificity

Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

Celluar localization

Endosome;Plasma Membrane;

P04629