Rabbit Anti-BCKD Polyclonal Antibody #abs130250

abs130250-50ug

1-2 Weeks

201

abs130250-100ug

1-2 Weeks

301

Description

BCKDK is an unusual protein kinase primarily associated with the mitochondrial matrix. It is characterized by containing a HATPase_c domain, a common feature found in various ATP-binding proteins such as protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. However, unlike PHKs, the BCK protein forms dimers by directly interacting the opposing nucleotide-binding domains. This distinctive characteristic sets BCKDK apart in its mode of dimerization compared to other related kinases.

Other names

Mitochondrial [lipoamide] dehydrogenase kinase, also known as BCKD kinase or BDK, is an enzyme involved in the regulation of the [3-methyl-2-oxobutanoate] dehydrogenase complex. This kinase plays a crucial role in the phosphorylation and activation of the mitochondrial branched-chain alpha-keto acid dehydrogenase enzyme. Its primary function is to control the activity of this enzyme complex, which is responsible for the breakdown of branched-chain amino acids in the mitochondria. The BCKD kinase ensures that the enzyme complex is appropriately regulated, thereby maintaining proper amino acid metabolism and energy production. Its significance in mitochondrial function makes it an important target for research in the field of metabolism and metabolic disorders.

Source

Rabbit

Specificity

The BCKD antibody is capable of detecting the overall levels of endogenous BCKD in the body. This means that the antibody binds specifically to BCKD proteins and can be used to measure their expression levels in cells or tissues. By detecting the total amount of BCKD present, researchers can gain insights into the metabolic pathways and pathways that are affected by BCKD. This type of antibody is an important tool in biomedical research, especially in the study of metabolic disorders such as maple syrup urine disease.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine

Application

The recommended dilutions for using the antibody in different applications are as follows: for Western blotting, a dilution range of 1:500~1:3000 is suggested. For immunofluorescence (IF) and immunocytochemistry (ICC), a dilution of 1:100~1:500 is recommended. For ELISA assays using peptides, a dilution range of 1:20000-1:40000 is recommended. It is important to follow the recommended dilution range to ensure optimal results when using this antibody.

Immunogen

A synthesized peptide derived from human BCKD.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum. Please generate a highly similar content by rearranging the above content, ensuring that the generated content is based on the original text information.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of your product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated cycles of freezing and thawing, as this can have a detrimental effect on its quality. So make sure to follow these guidelines to maintain the integrity of your product.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It should be stored at -20 °C and is stable for a period of 12 months from the date of receipt. If you're looking for a reliable and long-lasting source of Rabbit IgG, then this product is a great choice. It is designed to deliver consistent performance and is ideal for a wide range of research applications. Whether you're interested in basic immunology studies or more advanced projects, this product is sure to meet your needs. So if you want reliable Rabbit IgG at an affordable price, don't hesitate to try this product today!

Target

Background

The main function of this enzyme is to facilitate the phosphorylation and subsequent inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, which is responsible for regulating the catabolic pathways of valine, leucine and isoleucine. As a key regulatory enzyme, it plays a pivotal role in controlling the activity state of the BCKD complex.

Tissue specificity

Ubiquitous.

Posttranslational modification

Autophosphorylated.

Celluar localization

Mitochondrion;

UniPort

O14874