Phospho-FGFR1 (Tyr766) Rabbit Polyclonal Antibody#abs130784

abs130784-50ug

1-2 Weeks

201

abs130784-100ug

1-2 Weeks

301

Description

The fibroblast growth factor receptor family comprises proteins encoded by various genes. These receptors exhibit a significant conservation of amino acid sequence not only among themselves but also across different species throughout evolution. Although they share similar sequences, FGFR family members display variations in their affinity towards ligands and their distribution within tissues.

Other names

The basic fibroblast growth factor receptor 1 is also known as bFGF-R-1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR-1, FGFR1, and FGFR1/PLAG1 fusion. It is a protein that plays a key role in signal transduction pathways that are important in normal cell development and growth. The receptor binds to fibroblast growth factors (FGFs) and regulates a wide range of cellular processes, including cell division, differentiation, and migration.
Other names for this protein include FLG, FLT-2, FLT2, Fms-like gene, Fms-like tyrosine kinase 2, fms-related tyrosine kinase 2, HBGFR, heparin-binding growth factor receptor, HH2, HRTFDS, hydroxyaryl-protein kinase, KAL2, N-SAM, OGD, and Proto-oncogene c-Fgr. Understanding the role of this receptor is important in many areas of research, including cancer biology, developmental biology, and tissue engineering.

Source

Rabbit

Specificity

The Phospho-FGFR1 (Tyr766) Antibody is specifically designed to recognize and detect FGFR1 protein levels in cells and tissues when it is phosphorylated at Tyrosine 766. This antibody is highly selective and does not bind to unphosphorylated FGFR1, making it an excellent tool for studying protein function and signaling pathways. When applied to various experimental techniques, this antibody can provide valuable insights into the mechanisms behind various biological processes. Overall, Phospho-FGFR1 (Tyr766) Antibody is a powerful research tool that can aid in the understanding of cellular signaling and disease development.

Predictive reaction species

Rabbit;Sheep;Bovine;Horse;

Application

Our recommended dilution range for this protein is WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. It is important to note that these dilution ranges are based on the original information given. We highly suggest following our recommended dilutions to ensure optimal results for your experiments.

Tyrosine 766 is a crucial phosphorylation site in human FGFR1, and a synthesized peptide has been derived from this region. Let's create a highly similar content by rearranging the given information. However, please note that I'll generate text using a language model that may produce substantially different content.

Properties

1mg/ml

Purification

Purified rabbit serum was used to extract the antibody through a sequential chromatography process. The purification involved passing the serum through both phospho- and non-phospho-peptide affinity columns. It ensured that the resulting antibody was highly specific and of high quality.

Clonality

Polyclonal Antibody

To preserve the product's quality, it is recommended to store it at a temperature of -20 °C for up to 12 months. It's important to avoid exposing the product to repeated cycles of freezing and thawing. By following these storage guidelines, you can ensure that the product remains effective and can be used as intended.

Storage buffer

This solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, 150mM NaCl, and 0.02% sodium azide, along with 50% glycerol. It is recommended to store this solution at a temperature of -20°C, and it is expected to remain stable for up to 12 months from the date of receipt.

Target

Background

 Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

Tissue specificity

Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

Celluar localization

Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

P11362