Rabbit Anti-SUPT16H Polyclonal Antibody #abs130575

abs130575-50ug

1-2 Weeks

201

abs130575-100ug

1-2 Weeks

301

Description

The process of transcribing protein-coding genes can be carried out effectively on naked DNA with only the involvement of general transcription factors and RNA polymerase II. However, when DNA is packed into chromatin, transcription cannot occur without the aid of accessory factors. One such crucial factor is called FACT, which stands for "facilitates chromatin transcription." FACT has the ability to interact specifically with histones H2A/H2B, which helps to disassemble nucleosomes and allows for smooth transcription elongation. The two main subunits of FACT are the 80 kDa and 140 kDa proteins. The latter subunit is the focal point of the gene that encodes for FACT. Through its unique functions, FACT plays an essential role in promoting proper gene transcription on chromatinized DNA.

Other names

Chromatin-specific transcription elongation factor 140 kDa subunit, also known as CDC68, is an important component of the chromatin transcription complex. This 140 kDa subunit, together with the chromatin-specific transcription elongation factor, plays a crucial role in facilitating transcriptional processes. It is also referred to as the FACT 140 kDa subunit or FACTp140.
The protein encoded by the CDC68 gene is highly conserved and is a homolog of the Suppressor of Ty 16 protein. It is an essential factor for efficient transcription elongation through chromatin. The CDC68 protein is involved in regulating gene expression, chromatin remodeling, and modulating the structure of nucleosomes during transcription.
In addition, CDC68 is a subunit of the FACT complex, which stands for Facilitates Chromatin Transcription complex. The FACT complex is responsible for promoting transcription elongation by destabilizing nucleosomes in the path of RNA polymerase II. It acts as a molecular motor that moves along the DNA template, allowing RNA polymerase II to transcribe genes efficiently.
The function of CDC68, also known as hSPT16, is crucial for proper transcriptional regulation and chromatin maintenance. It interacts with other components of the transcriptional machinery to ensure accurate and efficient gene expression.
Therefore, CDC68, or Supt16h, plays a vital role in chromatin-specific transcription elongation and is an essential component of the FACT complex. Its proper functioning is crucial for maintaining the integrity and regulation of gene expression in cells.

Source

Rabbit

Specificity

The SUPT16H Antibody recognizes SUPT16H at its natural levels.

Species Reactivity

Human;Mouse

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

：
- ，1:500-1:2000。
- /，1:100-1:500。
- (ELISA)，1:20000-1:40000。
，。

Immunogen

A synthesized peptide derived from human SUPT16H.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the product's integrity, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can compromise its quality.

Storage buffer

The recommended storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to store the product at this temperature to maintain its stability for up to 12 months from the date of receipt.

Target

Background

The FACT complex, a vital component, plays a crucial role in chromatin reorganization by acting as a general factor. It is actively involved in numerous DNA-dependent processes such as DNA replication, mRNA elongation, and DNA repair. Specifically during transcription elongation, the FACT complex acts as a histone chaperone, effectively disrupting and then restoring the structure of nucleosomes. This enables the smooth passage of RNA polymerase II and facilitates transcription by promoting the dissociation of a histone H2A-H2B dimer from the nucleosome. Furthermore, it subsequently aids in the reestablishment of the nucleosome after RNA polymerase II has passed through. There is a possibility that the FACT complex is also associated with CK2 (casein kinase II), leading to the phosphorylation of 'Ser-392' residue on p53/TP53.

Tissue specificity

Ubiquitous.

Posttranslational modification

ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.

Celluar localization

Nucleus;

UniPort

Q9Y5B9