Rabbit Anti-Brg1 Polyclonal Antibody#abs131160

abs131160-50ug

1-2 Weeks

201

abs131160-100ug

1-2 Weeks

301

Description

This transcriptional coactivator works alongside nuclear hormone receptors to enhance transcriptional activation. It forms part of the CREST-BRG1 complex, which is a multiprotein complex involved in regulating promoter activation. This complex coordinates the release of a repressor complex and the recruitment of an activator complex in a calcium-dependent manner. Resting neurons inhibit transcription of the c-FOS promoter by employing the BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. However, calcium influx leads to the dephosphorylation of RB1 by calcineurin, resulting in the release of the repressor complex.

Other names

The ATP-dependent helicase SMARCA4, also known as BAF190, plays a crucial role in regulating gene expression by modifying chromatin structure. This protein, also referred to as BRG1, is a member of the SWI/SNF family of proteins and is involved in many biological processes such as cell proliferation, differentiation, and development. It has been identified as a key player in homeotic gene regulation and mitotic growth and transcription activation.
The SMARCA4 protein is encoded by the SMARCA4 gene and is a highly conserved protein among various species. It is composed of several functional domains, including an ATPase domain, a DNA-binding domain, and a helicase domain, which allow it to unwind and remodel chromatin structure in an ATP-dependent manner.
Studies have shown that the SMARCA4 protein is involved in various types of cancer, and its expression is often dysregulated in cancer cells. It has been suggested that targeting SMARCA4 could be a potential therapeutic strategy for cancer treatment.
Overall, the ATP-dependent helicase SMARCA4 is a critical protein that plays a fundamental role in regulating gene expression by altering chromatin structure. Its dysregulation has been associated with several diseases, and targeting it could be a promising therapeutic strategy in the future.

Source

Rabbit

Specificity

The total Brg1 protein's endogenous level is detected by the antibody. Please provide a content that closely resembles the original text but is rearranged to maintain the information.

Application

，WB1:500-1:1000，(ELISA)1:20000-1:40000。。

One possible rephrased version could be:
The peptide composition consisting of amino acids 701 to 705 (V-D-A-R-H) originated from the Human Brg1 protein. To create a closely related content, the arrangement of these amino acids could be altered while maintaining the same overall information.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Target

Background

 Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.

Tissue specificity

Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).

Celluar localization

P51532