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Rabbit Anti-GRB7 Polyclonal Antibody#abs137569

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our sales representative, Vecent. Western blot analysis GRB7 using COLO205 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs137569-100ug	1-2 Weeks	301.0
abs137569-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our sales representative, Vecent.

Overview
catalog	abs137569
Other names	GRB-7, also known as growth factor receptor-bound protein 7 or GRB7 adapter protein, is an important protein involved in the signaling pathway of epidermal growth factor receptor (EGFR). It serves as a key mediator between EGFR and downstream signaling molecules. The GRB7 protein is encoded by the GRB7 gene in humans and is commonly referred to as GRB7_HUMAN in scientific literature. Its function is to facilitate the transmission of signals from the activated EGFR to various cellular processes. The epidermal growth factor receptor (EGFR) is a transmembrane receptor that plays a critical role in cellular growth, proliferation, and differentiation. Upon binding to its ligands, EGFR undergoes a conformational change, leading to the activation of its intrinsic tyrosine kinase activity. This kinase activity subsequently triggers the recruitment of several adaptor proteins, including GRB7, to the EGFR signaling complex. GRB7 consists of several distinct domains, including a proline-rich region, a pleckstrin homology (PH) domain, and a Src homology 2 (SH2) domain. These domains enable GRB7 to interact with various signaling molecules and facilitate the formation of multiprotein complexes. One of the prominent binding partners of GRB7 is the phosphorylated tyrosine residues on activated EGFR. Through its interactions with EGFR, GRB7 plays a crucial role in regulating downstream signaling pathways, such as the MAPK/ERK and PI3K/AKT cascades. These pathways control diverse cellular processes, such as cell survival, proliferation, and migration. Moreover, GRB7 has been implicated in promoting breast cancer progression and metastasis. Increased expression of GRB7 has been observed in breast tumors, and its overexpression correlates with poor patient prognosis. In summary, GRB7 is a critical adaptor protein involved in EGFR signaling. Its interactions with activated EGFR facilitate the transmission of signals to downstream effectors, ultimately regulating crucial cellular processes. Further research on the precise mechanisms underlying the function of GRB7 may provide insights into the development of novel therapeutic strategies for cancer and other diseases involving EGFR dysregulation.
Source	Rabbit
Specificity	The GRB7 Antibody has the ability to identify total GRB7 levels present within an organism. This detection method targets endogenous sources of GRB7, ensuring accuracy in identifying the protein. To produce a highly similar content, the original text information was rearranged without changing its meaning.
Species Reactivity	Human;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;Horse
Antigen	GRB7
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human GRB7.
MW	60 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. It was an efficient process that allowed for the isolation of a highly concentrated and pure product.
Clonality	Polyclonal Antibody
Stability & Storage	For a duration of one year, it is recommended to store the item at a temperature of -20 °C. It is important to avoid subjecting the item to multiple cycles of freezing and thawing.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that the stability of the IgG is guaranteed for 12 months from the date of receipt.
Target
Background	Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).
Posttranslational modification	Phosphorylated on serine and threonine residues in response to heregulin. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues in response to NTN1 signaling. Phosphorylation promotes stress granule disassembly during recovery after cellular stress (By similarity). Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases. Tyrosine phosphorylation is essential for activation of down-stream protein kinases.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	Q14451