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Phospho-MLC-2 (Thr17+Ser18) Rabbit Polyclonal Antibody#abs139839

Phospho-MLC-2 (Thr17+Ser18) Rabbit Polyclonal Antibody#abs139839

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis MLC-2 (Phospho-Thr17+Ser18) using HepG2 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specificationDelivery timeUSD price

abs139839-100ug

1-2 Weeks

301.0

abs139839-50ug

1-2 Weeks

201.0

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.


Overview

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abs139839

Description

Myosin is a protein complex made up of six polypeptide chains. The complex features two heavy chains that are identical, and two pairs of light chains. Myosin light chain 2 or MLC2 is also known as myosin regulatory light chain (MRLC), RLC, or LC20, depending on its distribution and the type of muscle it is present in.
In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. This phosphorylation has been linked to myosin ATPase activity and smooth muscle contraction. When Ser19 of smooth muscle MLC2 is phosphorylated by ROCK, it regulates the assembly of stress fibers. PKC and cdc2 have been shown to phosphorylate Ser1/Ser2 and Ser9 of smooth muscle MLC2, respectively, causing the inhibition of myosin ATPase activity. CDC2's role in phosphorylating MLC2 also is responsible for controlling the timing of cytokinesis.
Transgenic mice without phosphorylation sites on the cardiac muscle isoform exhibited morphological and functional abnormalities which illustrate the importance of phosphorylation for proper muscle function. In summary, phosphorylation plays a crucial role in the regulation of myosin ATPase activity, muscle contraction, and cytokinesis, making it an essential mechanism in muscle function.

Other namesRegulatory myosin light chain 2 (MLC2) is a protein with a molecular weight of 20 kDa. It is encoded by the complete coding sequence of the human 20kDa myosin light chain (MLC2) mRNA. This protein is also known by various names such as LC20, MGC3505, MLC 2, MLC-2C, MLC2, MLY 9, MRLC1, MYL9, MYL9_HUMAN, Myosin light chain 9 regulatory, Myosin light polypeptide 9 regulatory, myosin regulatory light chain 1, Myosin regulatory light chain 2, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, Myosin RLC, Myosin vascular smooth muscle light chain 2, MYRL2, OTTHUMP00000030857, smooth muscle isoform, ML12B_HUMAN, MLC 2A, MLC-2, MLC-2a, MLC-B, MLC20, MRLC2, MYL12B, MYLC2B, Myosin light chain 12B regulatory, Myosin regulatory light chain 12B, myosin regulatory light chain 2, Myosin regulatory light chain 2-B, myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain 20 kDa, and Myosin regulatory light chain MRLC2.
SourceRabbit
SpecificityThe Phospho-MRLC1 (Thr18+Ser19) Antibody specifically recognizes phosphorylated MRLC1 at Thr18+Ser19, indicating endogenous levels of the protein. No detection of non-phosphorylated MRLC1 is evident. By rearranging the original information, a similar message is conveyed, emphasizing the importance of phosphorylation at these specific sites for recognition by the antibody.
Species ReactivityHuman;Mouse;Rat
Predictive reaction speciesXenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
AntigenMLC-2
ApplicationWB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
ImmunogenA synthesized peptide derived from human Phospho-MRLC1 (Thr18+Ser19) .
MW18kDa
Properties

Concentration

1mg/ml

purificationThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
ClonalityPolyclonal Antibody
Stability & StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage bufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).

Posttranslational modificationPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity).
Celluar localizationCytoskeleton;Cytosol;Extracellular region or secreted;
UniPortP19105/O14950/P24844


Western blot analysis MLC-2 (Phospho-Thr17+Ser18) using HepG2 whole cell lysates


This product is for research use only, not for use in diagnostic prodecures or in human.


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