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Rabbit Anti-Dematin Polyclonal Antibody#abs131504

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Description

Catalog-specification	Delivery time	USD price
abs131504-100ug	1-2 Weeks	301.0
abs131504-50ug	1-2 Weeks	201.0

Overview
catalog	abs131504
Description	This is a protein that has the function of bundling actin. It could possibly play a part in the mitogen-activated protein kinase pathway. It is found in various tissues such as the heart, brain, lung, skeletal muscle, and kidney.
Other names	DEMA, also known as DEMA_HUMAN, Dematin, DMT, EPB49, or erythrocyte membrane protein band 4.9, plays a crucial role in maintaining the integrity and stability of erythrocyte membranes. This protein, often referred to as erythrocyte membrane protein band 4.9, is essential for the proper functioning of red blood cells. Through rearranging the provided information, a new content is generated to emphasize the significance of DEMA in preserving the structure of erythrocyte membranes and facilitating normal erythrocyte function.
Source	Rabbit
Specificity	The Dematin Antibody can detect the total Dematin levels present within the organism. This indicates its ability to identify the endogenous levels of Dematin through its interactions with the biological system.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	Dematin
Application	The recommended dilutions for using a particular antibody may vary depending on the application. For example, with Western blotting, a typical dilution range is between 1:500 and 1:2000. Immunohistochemistry typically requires a more diluted antibody, between 1:50 and 1:200. When using the antibody in an immunofluorescence assay, a dilution factor between 1:100 and 1:500 is generally recommended. Lastly, in ELISA assays using peptides, the antibody may need to be diluted more starkly, typically between 1:20,000 and 1:40,000 for optimal results. These optimized antibody dilutions can help make your experiment more efficient and reliable with clear, reproducible results.
Immunogen	A synthesized peptide derived from human Dematin.
MW	45 kDa
Properties
Concentration	1mg/ml
purification	To obtain a pure antiserum, peptide affinity chromatography was employed using SulfoLink™ Coupling Resin as the chromatographic matrix.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, it is recommended to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the item to repeated freeze and thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation.
Tissue specificity	Expressed in platelets (at protein level). Expressed in heart, brain, lung, skeletal muscle, and kidney.
Posttranslational modification	Phosphorylated. Phosphorylation at Ser-403 by PKA causes the C-terminal headpiece domain to associate with the N-terminal core domain, and leads to the inhibition of its actin bundling activity.The N-terminus is blocked.
Celluar localization	Cytoskeleton;Cytosol;Plasma Membrane;
UniPort	Q08495