Phospho-p62 Dok (Tyr362) Rabbit Polyclonal Antibody#abs131103

abs131103-50ug

1-2 Weeks

201

abs131103-100ug

1-2 Weeks

301

Description

Dok1, a docking protein, exhibits its interaction with receptor tyrosine kinases. In chronic myelogenous leukemia (CML) patients during the chronic phase, it is continuously phosphorylated on tyrosine residues in hematopoietic progenitors. Let's generate a highly similar content by rearranging the provided information while ensuring its accuracy and consistency.

Other names

The docking protein 1 of 62kD, also known as DOK 1 or downstream of tyrosine kinase 1, is a vital protein involved in cellular signaling pathways. It is commonly referred to as p62(dok) or pp62 and plays a significant role in regulating cell growth, differentiation, and survival. With its ability to bind to various tyrosine-phosphorylated proteins, docking protein 1 acts as a molecular scaffold for downstream signaling molecules, leading to the activation of specific signaling pathways. This protein is highly conserved, and its dysregulation is often associated with several diseases, including cancer and autoimmune diseases. Overall, the significance of docking protein 1 in cellular signaling makes it an essential component of various physiological processes.

Source

Rabbit

Specificity

The Phospho-p62 Dok (Tyr362) Antibody is designed to specifically detect phosphorylated p62 Dok at Tyrosine 362. It does not recognize non-phosphorylated p62 Dok or other variants. This antibody is capable of detecting endogenous levels of p62 Dok only when it is phosphorylated at Tyrosine 362. It is a reliable tool for researchers looking to study the role of p62 Dok in various biological processes.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

The dilution ranges for WB are 1:500-1:2000, for IF/ICC are 1:100-1:500, and for ELISA with peptide are 1:20000-1:40000. It is essential to rearrange the content in a highly similar manner to retain the original text information.

A peptide obtained from Tyrosine 362 phosphorylation site within human p62 Dok has been synthesized. I request you to kindly generate content that closely resembles the original information, but please avoid using the ChapGPT conversation style. Instead, provide a completely different way of speaking based on the language model generated text.

Properties

1mg/ml

Purification

The antibody was obtained through a process of affinity purification using phospho- and non-phospho-peptide affinity columns on purified rabbit serum. The resulting product is highly specific and effective in targeting the intended antigen. Through this rigorous purification process, we were able to ensure the quality and purity of the antibody, providing confidence in its use for various applications.

Clonality

Polyclonal Antibody

It is recommended to store the product at -20 °C for a duration of one year. To maintain its quality, it is advised to prevent any repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, and 0.02% sodium azide. Additionally, the solution contains 50% glycerol as a stabilizer for storage at -20°C. This antibody is expected to remain stable for up to 12 months from the date of receipt.

Target

Background

DOK proteins serve as non-enzymatic adaptor or scaffolding proteins, playing a crucial role in organizing and facilitating the formation of complex signaling networks. Their primary function is to provide a platform for the assembly of multimolecular signaling complexes. Among these proteins, DOK1 stands out as a negative regulator of the insulin signaling pathway. It exerts its regulatory effect by modulating the activation of integrins, which are cell surface receptors involved in various cellular processes. Specifically, DOK1 competes with talin, another protein, for binding to the same site on the integrin subunit ITGB3. This competition ultimately influences the activation and function of integrins, further influencing cellular signaling events.

Tissue specificity

Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.

Celluar localization

Cytosol;Nucleus;

Q99704