Rabbit Anti-MMP16 Polyclonal Antibody #abs130150

abs130150-50ug

1-2 Weeks

201

abs130150-100ug

1-2 Weeks

301

Description

MMP16 is an endopeptidase that plays a significant role in degrading various components of the extracellular matrix. It specifically targets collagen type III and fibronectin, which are essential for maintaining the structural integrity of tissues. Additionally, this enzyme activates progelatinase A, further contributing to the remodeling of the matrix in blood vessels. Interestingly, there are different isoforms of MMP16, with the short isoform specifically cleaving fibronectin and collagen type III, albeit at a lower rate compared to the full-length isoform. Importantly, this enzyme does not have any effect on the degradation of collagen type I, II, IV, and V. This information highlights the diverse and specific role of MMP16 in mediating the remodeling process of the extracellular matrix in different physiological contexts.

Other names

C8orf57, also known as MMP16 or membrane type 3 matrix metalloproteinase, is a putative transmembrane protein that plays a pivotal role in various biological processes such as tissue remodeling and wound healing. MMP16 belongs to the family of matrix metalloproteinases which are zinc-dependent enzymes that degrade extracellular matrix proteins. MMP16 is also referred to as MT-MMP3, MT-MMP2, MT3-MMP, or MT3MMP, and is known to be highly expressed in cancer cell lines, particularly in breast and ovarian cancer. The overexpression of MMP16 has been linked to the promotion of tumor growth and metastasis by facilitating cancer cell invasion and migration. MMP16 is considered a potential therapeutic target for the treatment of cancer and other pathologies associated with abnormal matrix remodeling.

Source

Rabbit

Specificity

The MMP16 antibody is able to recognize and target total MMP16 levels present within the body. This means that it is capable of accurately detecting the endogenous amounts of MMP16 that are naturally produced within the system. By identifying this specific protein, the antibody can aid in various research and diagnostic applications, such as identifying potential disease markers and monitoring treatment progress. Overall, the MMP16 antibody serves as a valuable tool in studying the functions and effects of MMP16 in different biological contexts.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

Here are the recommended dilutions for different experimental techniques:
- For Western blotting (WB), the suggested dilutions range from 1:500 to 1:3000.
- For immunohistochemistry (IHC), it is advised to dilute the antibody at 1:50 to 1:200.
- If you are performing immunofluorescence or immunocytochemistry (IF/ICC), the optimal dilutions would be between 1:100 and 1:500.
- For ELISA using peptide antigens, the recommended dilutions are 1:20000 to 1:40000.
Please note that these dilution ranges ensure appropriate detection and minimal background signal.

Immunogen

A synthesized peptide derived from human MMP16.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. The resulting product was highly purified and ready for use in its intended applications.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repetitive freeze/thaw cycles.

Storage buffer

This product consists of Rabbit IgG that has been formulated in phosphate buffered saline at pH 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store this product at a temperature of -20°C and it is expected to remain stable for a period of 12 months from the date of receipt. Please note that this information has been rearranged from the original text in order to create a highly similar content.

Target

Background

This particular endopeptidase is responsible for breaking down various components of the extracellular matrix, such as collagen type III and fibronectin. Its main role is in the matrix remodeling of blood vessels. When activated, it also helps in the activation of progelatinase A. Interestingly, there is a shorter isoform of this enzyme that specifically cleaves fibronectin and collagen type III, albeit at a slower rate compared to the full-length isoform. It is important to note that this enzyme does not have any impact on collagen types I, II, IV, and V. However, when it interacts with CSPG4, it may play a role in the degradation and invasion of type I collagen by melanoma cells. Overall, this endopeptidase plays a significant part in the regulation and modification of the extracellular matrix.

Tissue specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Posttranslational modification

The precursor is cleaved by a furin endopeptidase.

Celluar localization

Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

UniPort

P51512