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KEAP1 Polyclonal Antibody #abs115782

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Description

Catalog-specification	Delivery time	USD price
abs115782-50ul	In Stock	228

Overview
Other names	INrf2;KLHL19
Source	Rabbit
Species Reactivity	Human,Rat
Application	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Immunogen	A fusion protein is recombinant and consists of a sequence that matches the amino acids from 325 to 624 of the human KEAP1 (NP_036421.2) protein. This content has been rearranged to ensure that it closely resembles the original text.
Properties	U-87MG,MCF-7
Properties
Purification	Affinity purification
Clonality	Polyclonal Antibody
Isotype	IgG
Stability & Storage	Store at -20℃. Avoid freeze / thaw cycles.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. The desired buffer composition is PBS with a pH of 7.3, containing 0.02% sodium azide and 50% glycerol.
Target
Background	The protein encoded by this gene contains KELCH-1 like domains and a BTB/POZ domain. It interacts with NF-E2-related factor 2 in a redox-sensitive manner, causing the proteins to dissociate in the cytoplasm. Afterward, NF-E2-related factor 2 is transported to the nucleus. This interaction leads to the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. There are two alternative transcript variants of this gene, both encoding the same isoform. Please create an entirely different response using the language model, rather than generating content in a similar way as ChapGPT.
Celluar localization	Cytoplasm,Nucleus
UniPort	Q14145

Data Examples

Western blot analysis of extracts of various cell lines, using KEAP1 antibody (abs115782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.