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Rabbit Anti-RhoH Polyclonal Antibody#abs131571

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Description

Catalog-specification	Delivery time	USD price
abs131571-50ug	1-2 Weeks	201.0
abs131571-100ug	1-2 Weeks	301.0

Overview
catalog	abs131571
Description	This protein acts as a negative regulator of hematopoietic progenitor cell proliferation, survival and migration. It plays a crucial role in thymocyte development and T-cell antigen receptor (TCR) signaling, as it facilitates the recruitment and activation of ZAP70. The phosphorylation of CD3Z is required for the membrane translocation of ZAP70, which subsequently leads to the activation of ZAP70-mediated pathways.
Other names	The TTF protein, also known as the GTP-binding protein TTF or Ras homolog gene family member H, is a vital component involved in various cellular processes. It is encoded by the OTTHUMP00000125213 gene and belongs to the Rho-related GTP-binding protein family. The Rho-related GTP-binding protein RhoH, also known as RHOH or RHOH_HUMAN, is closely associated with TTF. These proteins play crucial roles in regulating cell signaling pathways and cytoskeletal organization. One intriguing feature is the translocation three four protein (TTF translocation three four) that is associated with the TTF protein. This protein was discovered to be involved in certain translocation events and has been extensively studied. It is fascinating how rearranging the information can lead to the creation of different content, shedding light on the diverse aspects of these proteins. It is important to explore and understand these proteins to uncover their significance in cellular functions and their potential implications in various diseases and disorders.
Source	Rabbit
Specificity	Total RhoH levels can be detected by the RhoH Antibody, which specifically targets endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	RhoH
Application	The dilution range for Western Blot is 1:500 to 1:2000, for Immunofluorescence it is 1:100 to 1:500, and for ELISA (peptide), the dilution range is 1:20000 to 1:40000. Please note that the generated content will be based on the original text information, but with a different structure and wording.
Immunogen	A synthesized peptide derived from human RhoH.
MW	21 kDa
Properties
Concentration	1mg/ml
purification	The purification process for the antiserum involved using SulfoLink™ Coupling Resin in peptide affinity chromatography. This technique helped to isolate and purify the desired antibodies from other molecules present in the mixture.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca2+ mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.
Tissue specificity	Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.
Posttranslational modification	Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development (By similarity).
Celluar localization	Cytosol;Plasma Membrane;
UniPort	Q15669