Phospho-MITF (Ser180/73) Rabbit Polyclonal Antibody#abs130655

abs130655-50ug

1-2 Weeks

201

abs130655-100ug

1-2 Weeks

301

Description

MITF is a crucial transcription factor with unique structural features that have both basic helix-loop-helix and leucine zipper motifs. It plays a pivotal role in the differentiation of multiple cell types derived from neural crest, including melanocytes, mast cells, osteoclasts and retinal pigment epithelium developed from optic cups. MITF is essential for the proper functioning of these cell types and is involved in regulating numerous genetic pathways that influence cell growth, differentiation, and survival. Its importance in various physiological and pathological processes has made it a subject of interest among researchers studying developmental biology and cancer.

Other names

BHLHE32, also known as bHLHe32, is a protein belonging to the basic helix-loop-helix (bHLH) class E family. It is referred to as CMM8 and is the human homolog of the mouse microphthalmia gene. This gene is also known as Mi, Microphthalmia associated transcription factor, or MITF. Additionally, it is related to other names such as mitfa, nacre, WS2, WS2A, and z3A.1. By rearranging the provided information, I can create content that presents the same details in a different manner.

Source

Rabbit

Specificity

The Antibody for Phospho-MITF (Ser180/73) is capable of detecting MITF levels that are endogenous in nature. However, this detection is only possible when the MITF protein is phosphorylated at the specific Serine 180/73 site.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine

Application

ELISA (peptide) yields the highest dilution range of 1:20000-1:40000 compared to other techniques. IHC and IF/ICC have moderate dilution ranges of 1:50-1:200 and 1:100-1:500, respectively. WB has the widest dilution range from 1:500 to 1:2000. It is important to note that these dilution ranges are based on the original text information provided.

A peptide synthesized from the human MITF protein, specifically focused on the phosphorylation site of Serine 180/73, is currently being developed. By rearranging the provided information, the aim is to create new content that maintains its basis in the original text. This approach ensures a unique perspective rather than relying on the traditional dialogue structure employed by ChatGPT.

Properties

1mg/ml

Purification

The highly purified antibody was obtained by subjecting rabbit serum to affinity purification. This involved the use of phospho- and non-phospho-peptide affinity columns in sequential chromatography.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is advised to keep the product at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to multiple freeze-thaw cycles.

Storage buffer

The rabbit IgG is stored in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol at a temperature of -20°C. This storage condition is guaranteed for a period of 12 months from the receipt date. To maintain the quality of the antibody, it is important to follow the recommended storage instructions.

Target

Background

 Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Tissue specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

Celluar localization

Nucleus;

O75030