Phospho-ROCK2 (Tyr722) Rabbit Polyclonal Antibody#abs130656

abs130656-50ug

1-2 Weeks

201

abs130656-100ug

1-2 Weeks

301

Description

The gene in question encodes a kinase that belongs to the serine/threonine family. This kinase plays a crucial role in several physiological processes such as cytokinesis, actin stress fiber and focal adhesion formation, smooth muscle contraction, and activation of the c-fos serum response element. This protein is an isozyme of ROCK1, and it is regulated by the small GTPase Rho. [RefSeq provides this information]. As a result, this protein is an essential player in the regulation of numerous cellular and physiological processes.

Other names

The protein kinase 2 containing a coiled-coil domain is referred to as coiled-coil-containing protein kinase 2 or KIAA0619. It is also known as p164 ROCK 2 or p164 ROCK-2. This kinase is involved in various cellular processes and is classified under the name Rho associated coiled coil containing protein kinase 2. It is alternatively called Rho associated protein kinase 2 or Rho kinase 2. Another designation for this protein is Rho-associated, coiled coil containing protein kinase II. Additionally, it is known as ROCK 2, Rock II, Rock2, ROCK2_HUMAN, Rock2m, ROK alpha, or ROKalpha. By rearranging the content, we maintain the original information about this protein kinase and provide multiple alternative names for it.

Source

Rabbit

Specificity

Hu,Ms,Rt.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

（WB）1:2,000-5,000，（ELISA）1:20,000-1:40,000。。，。

A synthesized peptide derived from human ROCK2.

Properties

1mg/ml

Purification

The pure rabbit serum was used to extract the antibody through a series of chromatography processes involving both phospho- and non-phospho-peptide affinity columns. The resulting antibody is highly purified and closely resembles the original serum from which it was derived. This process ensures that the antibody is of high quality and suitable for various applications, including biological assays and medical research. Overall, the affinity purification technique is a reliable and effective method for obtaining antibodies with specific binding properties, and it plays a crucial role in the development of new therapeutic approaches.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

The rabbit IgG is suspended in a buffer containing phosphate and saline, with a pH of 7.4, along with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain its stability, it should be stored at -20 °C and can remain stable for up to 12 months from the date of receipt.

Target

Background

This protein kinase is a crucial regulator of actin cytoskeleton and cell polarity. Its functions include regulating smooth muscle contraction, organizing the actin cytoskeleton, forming stress fibers and focal adhesions, retracting neurites, and facilitating cell adhesion and motility. This is achieved through phosphorylation of various substrates, including ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A, and VIM. The kinase also phosphorylates SORL1 and IRF4 and negatively regulates VEGF-induced angiogenic endothelial cell activation. In addition, it plays a positive role in myogenic differentiation by activating p42/MAPK1-p44/MAPK3 and p90RSK/RPS6KA1. The kinase is critical for timely initiation of centrosome duplication and inhibits keratinocyte terminal differentiation. It may also regulate eyelid and ventral body wall closure and plays a vital role in spine and synaptic properties in the hippocampus. Finally, it modulates the myosin light chain phosphorylation to regulate the circadian rhythm of aortic myofilament Ca2+ sensitivity and vascular contractility.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;Plasma Membrane;

O75116