Phospho-MAP3K7 (Thr187) Rabbit Polyclonal Antibody#abs130647

abs130647-50ug

1-2 Weeks

201

abs130647-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Antibody to Phospho-MAP3K7 (Thr187) specifically targets the phosphorylated form of MAP3K7 at Threonine 187, allowing for the detection of endogenous levels of this protein. To provide a highly similar content based on the original text information, I can rearrange the sentences and maintain the core details as follows:
Detection of endogenous levels of MAP3K7 is enabled by the Phospho-MAP3K7 (Thr187) Antibody, which specifically recognizes the protein when it is phosphorylated at Threonine 187. This antibody's specificity allows for the identification of the phosphorylated form of MAP3K7, providing valuable insights into its cellular presence.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;

Application

For Western blotting, the recommended dilution range is 1:500 to 1:2000. For immunohistochemistry, the suggested dilution range is 1:50 to 1:500. For immunofluorescence/immunocytochemistry, the optimal dilution range is 1:100 to 1:500. For ELISA using a peptide substrate, the suggested dilution range is 1:20000 to 1:40000. It is important to dilute the samples properly to ensure accurate and reliable results in these assays.

A peptide synthesized from the human MAP3K7 protein, specifically targeting the phosphorylation site of Threonine 187, is now available. The peptide is highly similar to the original protein and offers a promising solution for research applications in various fields. So if you're looking for a reliable and effective peptide, this could be the perfect option for you.

Properties

1mg/ml

Purification

The antibody was obtained through purification of rabbit serum using a two-step affinity chromatography process involving phospho- and non-phospho-peptide affinity columns. This method allowed for the isolation of highly specific antibodies, which are crucial for accurate detection and quantification of target proteins. The affinity purification technique has been widely used in the production of high-quality antibodies for research and clinical applications.

Clonality

Polyclonal Antibody

To preserve the item's quality, it should be kept in a storage area with a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to multiple freeze/thaw cycles.

Storage buffer

The recommended storage conditions for Rabbit IgG include keeping it at -20 °C and storing it in phosphate buffered saline with a pH of 7.4. Additionally, it should be stored in a solution containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This storage arrangement allows for its stability for a period of 12 months starting from the date of receipt. It is crucial to adhere to these storage guidelines to ensure the quality and efficacy of the Rabbit IgG.

Target

Background

MAP3K7/TAK1 is a crucial serine/threonine kinase involved in the MAP kinase signaling pathway, which plays a vital role in cellular responses to environmental changes. It acts as a mediator for various cytokines such as interleukin-1 (IL-1), transforming growth factor-beta (TGFB), and TGFB-related factors like BMP2 and BMP4, as well as toll-like receptors (TLR), tumor necrosis factor receptor CD40, and B-cell receptor (BCR).
Activation of MAP3K7/TAK1 can also be triggered by ceramides. Once activated, it acts as an upstream activator for the MKK/JNK and p38 MAPK signal transduction cascades by phosphorylating and activating several MAP kinase kinases including MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7. These MAP2Ks then go on to activate p38 MAPKs, c-jun N-terminal kinases (JNKs), and the I-kappa-B kinase complex (IKK). The activation of AP-1 transcription factors is controlled by both p38 MAPK and JNK pathways, whereas nuclear factor-kappa B is activated by IKK.
In addition, MAP3K7/TAK1 is responsible for the activation of IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and also mediates BMP2-induced apoptosis. In osmotic stress signaling, it primarily activates MAPK8/JNK1, but not NF-kappa-B. Furthermore, it facilitates TRIM5 capsid-specific restriction activity.
By rearranging the provided information, the following content is generated:
MAP3K7/TAK1 serves as an integral serine/threonine kinase in the MAP kinase signal transduction pathway, playing a crucial role in the cellular responses triggered by changes in the surrounding environment. It functions as a key mediator in the signaling of TRAF6, various cytokines like interleukin-1 (IL-1) and transforming growth factor-beta (TGFB), as well as TGFB-related factors including BMP2 and BMP4. Toll-like receptors (TLR), tumor necrosis factor receptor CD40, and B-cell receptor (BCR) are also involved in its signal transduction.
Activation of MAP3K7/TAK1 can be initiated by ceramides, and once activated, it acts as an upstream activator for two significant signal transduction cascades: MKK/JNK and p38 MAPK. This activation occurs through the phosphorylation and activation of several MAP kinase kinases, specifically MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7. The activated MAP2Ks, in turn, activate p38 MAPKs, c-jun N-terminal kinases (JNKs), and the I-kappa-B kinase complex (IKK). These pathways control the activity of various transcription factors such as activator protein-1 (AP-1) and nuclear factor-kappa B.
Furthermore, MAP3K7/TAK1 is involved in the activation of IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and also plays a significant role in mediating BMP2-induced apoptosis. Although it primarily activates MAPK8/JNK1 in osmotic stress signaling, it does not participate in the activation of NF-kappa-B. Additionally, it promotes the capsid-specific restriction activity of TRIM5.
Please note that the rearranged content may not be an accurate representation of the original text. It is suggested to refer to the original text for precise information.

Tissue specificity

Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.

Celluar localization

Cytosol;Endosome;Nucleus;Plasma Membrane;

O43318