Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody#abs131076

abs131076-50ug

1-2 Weeks

201

abs131076-100ug

1-2 Weeks

301

Description

The SMAD gene encodes a protein that is a member of the SMAD protein family, which shares similarities with the Mad gene found in Drosophila and the Sma gene in C. elegans. These proteins function as transcriptional modulators and signal transducers that play a crucial role in multiple signaling pathways. As such, they are essential components in cellular communication and are involved in a wide range of biological processes.

Other names

BSP-1 is also known as BSP1, HsMAD1, JV4-1, JV41, or MAD homolog 1. It is a protein that belongs to the MAD family and is involved in the regulation of various cellular processes. MAD1 is a related protein that shares similarities with the Mothers against decapentaplegic homolog 1 (MADR1). It is also known as Mothers against DPP homolog 1, SMA- and MAD-related protein 1, or transforming growth factor-beta-signaling protein 1. Another name for the SMAD1 protein is SMAD family member 1. SMAD1 is a critical component of the TGF beta signaling pathway. It is essential for transmitting signals from the cell surface receptor to the nucleus, where it regulates gene expression.

Source

Rabbit

Specificity

Our Phospho-Smad1 (Ser465) Antibody is specifically designed to recognize and bind to Smad1 protein only when it is phosphorylated at Serine 465. This antibody is able to detect the endogenous levels of phosphorylated Smad1, providing valuable information for your research. By targeting this specific phosphorylation site, we can accurately assess the activation status of Smad1 in various cellular contexts.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Bovine;Sheep;Pig;

Application

The dilution ranges for WB (Western Blot) are 1:500-1:2000, for IHC (Immunohistochemistry) are 1:50-1:200, for IF/ICC (Immunofluorescence/Immunocytochemistry) are 1:100-1:500, and for ELISA (Enzyme-linked immunosorbent assay) using peptides are 1:20000-1:40000. These dilution ranges are important in determining the optimal concentrations for antibody detection in various experimental techniques.

One potential peptide to consider is a synthetic version of human Smad1, specifically targeting the phosphorylation site of Serine 465. This tailored peptide could offer valuable insights into processes such as cellular signaling and gene expression regulation, potentially leading to new therapeutic approaches for various diseases and conditions. By carefully designing and testing this peptide, researchers may be able to uncover previously unknown mechanisms and pathways related to Smad1 function that could have significant implications for human health.

Properties

1mg/ml

Purification

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns was utilized to purify the antibody from purified rabbit serum. The resulting antibody is highly specific and efficiently binds to both phospho- and non-phospho-peptides. This purification process ensures the isolation of a high-quality antibody that can be used for various applications in research and diagnostics.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated freeze and thaw cycles, which could potentially compromise its quality. Therefore, handle the product with care to maintain its integrity and long-term viability.

Storage buffer

Please note that the following information is a rearrangement of the original text.
To maintain the stability and integrity of rabbit IgG, it is recommended to store it at a temperature of -20 °C. The IgG is dissolved in phosphate buffered saline with a pH of 7.4 and contains 150mM NaCl. Additionally, the solution contains 0.02% sodium azide to prevent bacterial growth. To further enhance its stability, 50% glycerol is added. The rabbit IgG solution can be stored under these conditions for a duration of 12 months from the date of receipt.

Target

Background

 Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Tissue specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Celluar localization

Cytosol;Nucleus;

Q15797