Phospho-I KappaB- Beta (Ser23) Rabbit Polyclonal Antibody#abs131072

abs131072-50ug

1-2 Weeks

201

abs131072-100ug

1-2 Weeks

301

Description

kB-beta, a member of the NF-kappa-B inhibitor protein family, functions by inhibiting the activity of NF-kappa-B. It achieves this by forming a complex with NF-kappa-B, consequently trapping it within the cytoplasm. However, following cellular stimulation, the unphosphorylated form of kB-beta is synthesized anew, enabling it to bind to NF-kappa-B. This binding facilitates the transportation of NF-kappa-B to the nucleus and provides protection against further inactivation by the IKBA protein.

Other names

I-kappa-B-beta, also known as IKB beta or NF-kappa-B inhibitor beta, is a protein encoded by the IKBKB gene in humans. Other names include IkappaBbeta, IkB-B, IKBB, and NF kappa BIB. It is also referred to as Thyroid receptor-interacting protein 9 or TRIP-9 due to its interaction with the thyroid receptor. This protein plays a critical role as an inhibitor of the NF-kappa-B pathway, which regulates immune responses and inflammation. Dysregulation of NF-kappa-B activity has been implicated in various diseases, including cancer and autoimmune disorders. Understanding the function of I-kappa-B-beta is essential for developing therapeutic strategies to modulate NF-kappa-B activity and treat these conditions.

Source

Rabbit

Specificity

The detection capability of Phospho-I kappaB-beta (Ser23) Antibody is specific to the phosphorylated form of I kappaB-beta at Serine 23, allowing for accurate measurement of its endogenous levels. It is important to note that the antibody does not recognize or bind to the unphosphorylated I kappaB-beta.

Application

For Western blot, dilute the antibody at 1:500-1:2000. For immunohistochemistry, dilute at 1:50-1:200. For immunofluorescence and ICC, dilute at 1:100-1:500. For ELISA using peptide, dilute at 1:20000-1:40000. It's important to maintain these dilution ratios to ensure accurate results. Make sure to follow the instructions carefully for optimal performance of the antibody.

A peptide has been synthesized that is based on human I kappaB- beta and focuses on the phosphorylation site of Serine 23. The resulting peptide has a high degree of similarity to the original sequence and can be used in various research studies related to this protein. The synthesis process involved careful selection of amino acids to ensure that the resulting peptide had the desired properties and could accurately reflect the original protein sequence. This synthesized peptide represents an important tool for studying the functions and interactions of human I kappaB- beta, and may lead to new insights and discoveries in the field of molecular biology.

Properties

1mg/ml

Purification

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns was carried out to purify the antibody from purified rabbit serum.

Clonality

Polyclonal Antibody

To ensure the preservation of the product, it is recommended to store it at -20 °C for a period of one year. It is essential to prevent the occurrence of freeze/thaw cycles as they can have a detrimental effect on the quality of the item. Thus, maintaining a stable temperature throughout the storage duration is necessary.

Storage buffer

The solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4. It also includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature is -20 °C, and it can remain stable for 12 months starting from the date of receipt.

Target

Background

By forming a complex with NF-kappa-B and sequestering it in the cytoplasm, the activity of this protein is inhibited. However, following cell stimulation, the unphosphorylated form is regenerated and capable of binding to NF-kappa-B, allowing its transportation into the nucleus while also providing protection against further NFKBIA-dependent inactivation. This protein's resistance to degradation is increased when it is associated with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2, preventing phosphorylation and reducing the speed of degradation. Therefore, the slower degradation can be explained by its ability to resist degradation due to its association with these proteins.

Tissue specificity

Expressed in all tissues examined.

Celluar localization

Cytosol;Nucleus;

Q15653