Phospho-PPAR-BP (Thr1457) Rabbit Polyclonal Antibody#abs131071

abs131071-50ug

1-2 Weeks

201

abs1310715-100ug

1-2 Weeks

301

Description

Recognizing transcriptional enhancer sites in DNA, factors initiate the activation of gene transcription through a multistep process. Collaborating with co-activators, these factors guide the RNA polymerase II apparatus in initiating transcription. The gene's encoded protein acts as a subunit within the CRSP complex, known as a cofactor that facilitates SP1 activation. Efficient activation by SP1 also requires the presence of TFIID alongside the CRSP complex.

Other names

The Activator-recruited cofactor 205 kDa component, also known as ARC205, CRSP1, CRSP200, DRIP205, DRIP230, MED1, PBP, PPAR binding protein, and RB18A, is a crucial subunit of the Mediator complex that plays a critical role in RNA polymerase II transcription. This protein also acts as a regulatory factor for the tumor suppressor protein p53, highlighting its significance in cancer research. Additionally, it is a component of the thyroid hormone receptor-associated protein complex and the vitamin D receptor-interacting protein complex. Thyroid receptor-interacting protein 2, also known as TRIP-2 or TRIP2, is another name for this protein. Trap220, the largest subunit of the Mediator complex, is another of its aliases. This protein plays a vital role in a broad range of cellular processes, making it a promising target for therapeutic interventions in numerous diseases.

Source

Rabbit

Specificity

The antibody for Phospho-PPAR-BP (Thr1457) is capable of detecting PPAR-BP levels that are endogenous only when Threonine 1457 has been phosphorylated. To create similar content, the original text can be rearranged while still maintaining the same important points.

Predictive reaction species

Rabbit;Sheep;Dog;Bovine;Horse

Application

The recommended dilutions for using this antibody in Western blot are between 1:500 to 1:2000. For immunohistochemistry, dilutions of 1:50 to 1:200 are recommended, while for immunofluorescence and immunocytochemistry, a dilution range of 1:100 to 1:500 can be used. When using this antibody in ELISA (peptide), a dilution range of 1:20000 to 1:40000 is recommended. It is important to note that dilution recommendations may vary depending on the specific application and experimental conditions.

A peptide that has been created synthetically from human PPAR-BP and has been derived from the region surrounding Threonine 1457's phosphorylation site is under consideration. It is expected to have a high degree of similarity to the original sequence of the peptide.

Properties

1mg/ml

Purification

The affinity-purified antibody was derived from purified rabbit serum using sequential chromatography on columns of phospho- and non-phospho-peptide affinity.

Clonality

Polyclonal Antibody

To preserve its quality, it is advisable to store the material at -20 °C for up to a year. It is critical to avoid exposing the substance to repeated freeze/thaw cycles to prevent damage.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).

Tissue specificity

Ubiquitously expressed.

Celluar localization

Nucleus;

Q15648