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Phospho-PKCT (Tyr90) Rabbit Polyclonal Antibody#abs140012

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Description

Catalog-specification	Delivery time	USD price
abs140012-100ug	1-2 Weeks	301.0
abs140012-50ug	1-2 Weeks	201.0

Overview
catalog	abs140012
Other names	Protein kinase C theta, also known as nPKC-theta or PRKCT, is a type of protein kinase belonging to the protein kinase C family. It plays an important role in the immune system by regulating the activation, differentiation, and survival of T cells. nPKC-theta is found predominantly in T cells, where it is involved in the formation and function of the immunological synapse, a specialized junction between T cells and antigen-presenting cells. PRKCT has been identified as a potential therapeutic target for autoimmune diseases and cancer due to its critical role in immune responses and cell signaling pathways. Understanding the functions of Protein kinase C theta is essential in the development of novel therapies for various diseases.
Source	Rabbit
Specificity	The detection of endogenous levels of PKCT is possible with the Phospho-Tyr90 Antibody, specifically when the protein is phosphorylated at Tyr90. It is crucial to note that this antibody is designed to specifically target the phosphorylated form of PKCT at this particular site.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Bovine;Horse;
Antigen	PKCT
Application	ELISA (peptide) has a recommended dilution range of 1:20000 to 1:40000. For immunohistochemistry (IHC), the recommended dilution is 1:50 to 1:200. When it comes to immunofluorescence/immunocytochemistry (IF/ICC), the dilution range is 1:100 to 1:500. Lastly, for Western blotting (WB), the recommended dilution range is 1:1000 to 1:3000. These dilution suggestions should be followed to obtain accurate and reliable results.
Immunogen	A peptide synthesized from the Tyrosine 90-phosphorylated form of human PKCT has been developed. The structure of this peptide closely resembles that of the original protein, and may provide useful insights into the function of PKCT in biological processes. It is hoped that this peptide will prove to be a valuable tool for further research in the field of protein biochemistry.
MW	80kDa
Properties
Concentration	1mg/ml
purification	By employing sequential chromatography on phospho- and non-phospho-peptide affinity columns, the purified rabbit serum yields the antibody through an affinity purification process.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage, it is recommended that you keep the item in a freezer at a temperature of -20°C for up to one year. It is important to avoid subjecting the item to repeated freeze/thaw cycles, as this can compromise its quality. Making sure the item is properly stored will help to maintain its effectiveness over time.
Storage buffer	This rabbit IgG solution is composed of phosphate buffered saline at a pH of 7.4, with the addition of 150mM of NaCl and 0.02% sodium azide. It is also blended with 50% glycerol to ensure the stability of the product when stored at -20°C. This solution can be kept for up to 12 months from the date it was received.
Target
Background	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1.
Tissue specificity	Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.
Posttranslational modification	Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685.
Celluar localization	Cytoskeleton;Cytosol;Plasma Membrane;
UniPort	Q04759