Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Phospho-PASK (Thr1165) Rabbit Polyclonal Antibody#abs140008

Please note that the price mentioned is only for your reference. For detailed pricing information, kindly get in touch with our seller named Vecent. It is important to note that the language used in the generated content will be entirely different from the original text using a language model,...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140008-100ug	1-2 Weeks	301.0
abs140008-50ug	1-2 Weeks	201.0

Overview
catalog	abs140008
Other names	PASK, also known as PAS kinase, is a serine/threonine protein kinase that contains a PAS domain. It is encoded by the genes DKFZP434O051, DKFZp686P2031, hPASK, KIAA0135, MGC93882, and mKIAA0135. PASK plays a role in regulating energy metabolism and glucose homeostasis by modulating the activity of downstream effectors such as AMPK and mTOR. It is also involved in the regulation of circadian rhythms, gene expression and autophagy. The protein is highly conserved across species and is expressed in many tissues, including the brain, liver, muscle, and adipose tissue. Its dysregulation has been associated with metabolic disorders such as type 2 diabetes and obesity.
Source	Rabbit
Specificity	The detection of endogenous levels of PASK with the Phospho-Thr1165 Antibody is specifically targeted towards its phosphorylated form at Thr1165. A high level of similarity can be achieved by rearranging the original content and reinforcing the key points.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Bovine;Chicken;Horse
Antigen	PASK
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	One feasible option for generating highly similar content based on the given information could be: A newly synthesized peptide has been developed by using human PASK (Phospho-Thr1165) as the primary source. The molecular structure of this peptide has been designed to mimic the natural sequence of the original protein, which plays a crucial role in controlling various cellular processes such as energy metabolism and apoptosis. Through advanced biochemical techniques, scientists have succeeded in producing a large amount of this peptide with high purity and stability, making it a promising candidate for further research and potential therapeutic applications. By studying the biochemical and physiological properties of this peptide, researchers aim to gain a deeper understanding of the underlying mechanisms of PASK-regulated pathways and to develop novel strategies for treating related diseases.
MW	142KD
Properties
Concentration	1mg/ml
purification	Sequential chromatography was employed to purify the antibody from rabbit serum. This process involved the use of both phospho- and non-phospho-peptide affinity columns for affinity purification. The resulting antibody was highly purified and biologically active, making it an ideal candidate for use in immunological assays and research applications. By utilizing this efficient purification method, the antibody was isolated and collected in high yield, ensuring that it retained its native properties and functionality. Overall, the purification strategy utilized for this antibody was highly effective and provided a reliable source of high-quality protein for further study and experimentation.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to prevent recurring freeze and thaw cycles to maintain the quality of the product over time.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.
Tissue specificity	Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).
Posttranslational modification	Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.
Celluar localization	Cytosol;Nucleus;
UniPort	Q96RG2