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Rabbit Anti-JAK2 Polyclonal Antibody#abs131625

Description

Catalog-specification	Delivery time	USD price
abs131625-50ug	In Stock	201.0
abs131625-100ug	In Stock	301.0

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Overview
catalog	abs131625
Description	An integral component of cytokine receptor signaling pathways, this protein tyrosine kinase participates in a distinct subset. Its association with the prolactin receptor is constant and essential for eliciting responses to gamma interferon. Reordering the provided information, a similar content can be generated.
Other names	Here is a rearranged version of the content: Janus kinase 2 (JAK2), also known as JAK-2 or JAK2_HUMAN, is a protein tyrosine kinase. It plays a significant role in cellular signaling pathways. JAK2 is involved in the activation of Janus Activating Kinase 2 and is responsible for regulating various cellular processes. Its function as a tyrosine protein kinase makes it an important component in signal transduction pathways. JAK2 is also referred to as JTK10 or JTK 10. Other aliases include THCYT3, OTTHUMP00000043260, and Tyrosine-protein kinase JAK2. It is a versatile enzyme that plays a crucial role in cellular communication and signaling.
Source	Rabbit
Specificity	The total JAK2 levels can be accurately detected by using the JAK2 Antibody, which specifically recognizes the endogenous JAK2 protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	JAK2
Application	For Western blotting, the recommended dilution range is 1:500 to 1:2000. Immunohistochemistry requires a dilution range of 1:50 to 1:200, while for immunofluorescence and immunocytochemistry, the optimal dilution range is 1:100 to 1:500. The recommended dilution range for ELISA with peptide is 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human JAK2.
MW	125kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was employed to purify the antiserum, employing SulfoLink™ Coupling Resin as the method.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980).
Tissue specificity	Ubiquitously expressed throughout most tissues.
Posttranslational modification	Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558, PubMed:7673114).
Celluar localization	Cytoskeleton;Cytosol;Endosome;Nucleus;Plasma Membrane;
UniPort	O60674