Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-HMGB3 Polyclonal Antibody#abs137302

Please note that the price provided is for your reference only. For detailed pricing information, kindly contact our seller, Vecent. Feel free to reach out to Vecent for more accurate and up-to-date pricing details. Western blot analysis HMGB3 using COLO205 whole cell lysates This product is for...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs137302-100ug	1-2 Weeks	301.0
abs137302-50ug	1-2 Weeks	201.0

Overview
catalog	abs137302
Other names	HMG4, also known as high mobility group (nonhistone chromosomal) protein 4 or High Mobility Group Box 3, is a chromosomal protein that plays a crucial role in DNA packaging and gene regulation. It is a type of high mobility group (HMG) protein, specifically HMG2a. Other names associated with this protein include High Mobility Group Protein 4, High Mobility Group Protein B3, HMG-4, HMG2A, HMGB3, Hmgb3, HMGB3_HUMAN, MGC90319, Nonhistone Chromosomal Protein, and Nonhistone Chromosomal Protein HMG4. HMG4 is categorized as a nonhistone protein, meaning it is not involved in the formation of nucleosomes like histone proteins. Instead, it binds to DNA and helps in the organization and structure of chromatin. Its high mobility group box (HMGB) domains enable it to interact with DNA, bending and unwinding the double helix as necessary for various cellular processes. This protein is vital for DNA transactions, including transcription, replication, recombination, and repair. It influences gene expression by facilitating the assembly of transcription factor complexes and the recruitment of other proteins involved in gene regulation. HMG4 also participates in chromatin remodeling, modulating the accessibility of DNA to transcriptional machinery. The diverse range of names associated with HMG4 highlights the significance of this protein in multiple biological contexts. Understanding its functions and mechanisms of action can contribute to advancements in fields like genetics, epigenetics, and molecular biology. Further research exploring the roles of HMG4 may uncover its potential as a therapeutic target for various diseases and disorders.
Source	Rabbit
Specificity	Endogenous levels of total HMGB3 can be accurately detected using the HMGB3 Antibody.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	HMGB3
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human HMGB3.
MW	23 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was employed to purify the antiserum, utilizing SulfoLink™ Coupling Resin as the resin matrix.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimum storage conditions, it is recommended to keep the product at a temperature of -20°C for a period of one year. It is advisable to avoid subjecting the product to repeated cycles of freezing and thawing as this can compromise its quality. Therefore, take necessary precautions when storing the product to maintain its integrity for longer periods.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Tissue specificity	Expressed predominantly in placenta.
Posttranslational modification	Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).
Celluar localization	Nucleus;
UniPort	O15347