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Phospho-RAD9 (Ser272) Rabbit Polyclonal Antibody#abs139848

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Description

Catalog-specification	Delivery time	USD price
abs139848-100ug	1-2 Weeks	301.0
abs139848-50ug	1-2 Weeks	201.0

Overview
catalog	abs139848
Other names	The cell cycle is a highly regulated process that ensures proper cell division and replication. One important component of this process is the cell cycle checkpoint control protein RAD9A. This protein helps to monitor the DNA replication process and identify any errors or mutations that may have occurred. RAD9A is also known as the DNA repair exonuclease rad9 homolog A, hRAD 9, hRAD9, Rad 9, RAD 9A, RAD9 (S pombe) homolog, RAD9 homolog A, RAD9 homolog, and Rad9a. The role of RAD9A is critical to maintaining the integrity of the cell cycle. It acts as a gatekeeper at the G2/M checkpoint, allowing the cell to progress to the next stage of division only if DNA damage has been successfully repaired. RAD9A responds to DNA damage and replication stress by activating a cascade of checkpoint control proteins that halt the cell cycle until the damage is identified and repaired. In addition to its role in checkpoint control, RAD9A also plays a significant role in DNA repair. It functions as an exonuclease that can remove damaged DNA from the genome and facilitate repair mechanisms. The ability of RAD9A to recognize and repair DNA damage is crucial to preventing mutations, which can lead to harmful diseases like cancer. The importance of RAD9A in the cell cycle and DNA repair is underscored by its conservation across species. RAD9A is highly similar to the RAD9 protein in yeast, which has a similar function in DNA repair and checkpoint control. The homology between these proteins highlights the importance of maintaining proper DNA replication and repair mechanisms in all organisms.
Source	Rabbit
Specificity	The antibody RAD9 (Phospho-Ser272) is specifically designed to recognize and bind to RAD9 protein only when it is phosphorylated at Ser272. This antibody allows for the detection of endogenous levels of RAD9 protein that have undergone phosphorylation at this specific site.
Species Reactivity	Human
Antigen	RAD9
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	RAD9，Ser272。
MW	42547.0
Properties
Concentration	1mg/ml
purification	The purification process of the antibody involves sequential chromatography on phospho- and non-phospho-peptide affinity columns using purified rabbit serum. By utilizing this affinity purification technique, the antibody is extracted and separated from other components in the serum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, it is recommended to store the product at a temperature of -20°C for up to one year. Caution should be taken to avoid repeated cycles of freezing and thawing, as it may affect the product's effectiveness.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline (pH 7.4), 150mM NaCl, and 0.02% sodium azide, with 50% glycerol added as a stabilizer. It should be stored at -20°C and has a shelf life of 12 months from the date of receipt.
Target
Background	Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Posttranslational modification	Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Celluar localization	Nucleus;
UniPort	Q99638