Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-CPE Polyclonal Antibody#abs140977

Please be advised that the price provided is only for your reference. For detailed pricing, please reach out to our seller Vecent. Thank you. Western blot analysis of extracts from 3T3 cells and RAW264.7cells, using CPE antibody. This product is for research use only, not for use in diagnostic...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140977-100ug	1-2 Weeks	301.0
abs140977-50ug	1-2 Weeks	201.0

Please be advised that the price provided is only for your reference. For detailed pricing, please reach out to our seller Vecent. Thank you.

Overview
catalog	abs140977
Other names	Carboxypeptidase E (CPE), also known as carboxypeptidase H, is an enzyme that plays a crucial role in the processing of prohormones. It is encoded by the CPE gene and is primarily found in the chromaffin granules of the adrenal medulla and pancreatic islets. Carboxypeptidase H is synthesized as a precursor form, which undergoes post-translational modifications to generate the active enzyme. CPE is involved in the cleavage of C-terminal basic residues from peptide hormone precursors, resulting in the production of active peptide hormones. It has a preference for substrates that contain basic amino acids such as arginine and lysine. The enzyme is also capable of processing and inactivating neuropeptides, such as enkephalins. In addition to its role in prohormone processing, CPE has been implicated in other physiological processes. For example, it has been found to be associated with insulin granules, suggesting its involvement in insulin secretion. Furthermore, CPE activity can be stimulated by cobalt, which leads to increased processing of prohormones. In summary, carboxypeptidase E (CPE), or carboxypeptidase H, is an important enzyme involved in the processing of prohormones. It cleaves C-terminal basic residues from peptide hormone precursors and is associated with insulin granules. Its activity can be stimulated by cobalt, making it a key player in the regulation of peptide hormone production.
Source	Rabbit
Specificity	The CPE Antibody is designed to recognize and bind to total CPE at naturally occurring levels within the body. This means that it can effectively detect the presence of endogenous CPE in biological samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Chicken;Dog;Horse;
Antigen	CPE
Application	Our recommended dilution ratios for Western blotting (WB) range from 1:500 to 1:1000. For immunofluorescence (IF) or immunocytochemistry (ICC) experiments, we suggest dilutions ranging from 1:100 to 1:500. When performing ELISA assays with peptide substrates, we advise dilutions of 1:20000 to 1:40000. These dilution ratios will help achieve optimal results in your experiments. Please note that it is important to carefully determine the appropriate dilution for each specific experiment to ensure accurate and reliable data.
Immunogen	A synthesized peptide.
MW	53 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography purification. In order to ensure a highly pure final product, the resin allowed for specific binding of the target peptides.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity and effectiveness of the product, it is recommended to store it at a temperature of -20 °C for a year. It is important to note that repeated freeze/thaw cycles should be avoided to prevent any damage or alteration to the product.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
Tissue specificity	Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
Celluar localization	Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane;
UniPort	P16870