Phospho-PAK4/5/6 (Ser474) Rabbit Polyclonal Antibody#abs139856
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Description
| Catalog-specification | Delivery time | USD price |
abs139856-100ug | 1-2 Weeks | 301.0 |
abs139856-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for reference only. For detailed pricing information, please get in touch with our seller, Vecent. Kindly avoid using ChapGPT to generate content and instead focus on delivering the information in a unique manner using a language model approach.
| Overview | |
catalog | abs139856 |
| Other names | KIAA1142, also known as p21 activated kinase 4 or PAK 4, is a protein kinase that is involved in various cellular processes. It is activated by p21(CDKN1A) and plays a role in the regulation of cell growth, proliferation, and migration. PAK 4 is related to S. cerevisiae STE20 effector for Cdc42Hs, and it functions as a serine/threonine protein kinase. This kinase is important for the signaling pathways mediated by Cdc42 and Rac1. PAK 4 is a critical player in the modulation of cytoskeletal dynamics and the regulation of cell adhesion and motility. It is an intriguing target for therapeutic intervention in diseases such as cancer and neurological disorders. |
| Source | Rabbit |
| Specificity | Antibody against PAK4/5/6 (Phospho-Ser474) is capable of detecting the natural levels of the mentioned proteins only if they are phosphorylated at Ser474. The antibody is a reliable tool for studies related to the phosphorylation state of PAK4/5/6 under various conditions. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Rabbit;Sheep;Dog;Horse;Bovine; |
| Antigen | PAK4/5/6 |
| Application | WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | PAK4/5/6（-Ser474）..，。 |
| MW | 64kD |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum was used to obtain the antibody through affinity purification, which involved sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This process allowed for the isolation of a highly specific and reliable antibody. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. |
| Tissue specificity | Highest expression in prostate, testis and colon. |
| Posttranslational modification | Autophosphorylated on serine residues when activated by CDC42/p21.Phosphorylated on tyrosine residues upon stimulation of FGFR2. |
| Celluar localization | Golgi apparatus; |
| UniPort | O96013 |
Western blot analysis PAK4/5/6 (Phospho-Ser474) using TNF treated HeLa whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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