Phospho-PKAalpha CAT (Ser338) Rabbit Polyclonal Antibody#abs139846
Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller Vecent. We are here to assist you with any further queries or assistance you may require. Western blot analysis PKAα CAT (Phospho-Ser338)...
Description
| Catalog-specification | Delivery time | USD price |
abs139846-100ug | 1-2 Weeks | 301.0 |
abs139846-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller Vecent. We are here to assist you with any further queries or assistance you may require.
| Overview | |
catalog | abs139846 |
| Other names | There are several different forms of cAMP-dependent protein kinase catalytic subunits. These include cAMP-dependent protein kinase alpha catalytic subunit, cAMP-dependent protein kinase beta catalytic subunit, cAMP-dependent protein kinase catalytic beta subunit isoform 4ab, and cAMP-dependent protein kinase catalytic subunit alpha. Additionally, there are isoforms such as cAMP-dependent protein kinase catalytic subunit alpha isoform 1 and cAMP-dependent protein kinase catalytic subunit beta. Other names for these subunits include KAPCA_HUMAN, PKA C alpha, PKA C beta, PKA C-alpha, PKACA, PKACB, PPNAD4, PRKACA, PRKACAA, PRKACB, Protein kinase A catalytic subunit alpha, Protein kinase A catalytic subunit, Protein kinase A catalytic subunit beta, Protein kinase, cAMP dependent, catalytic, alpha, and Protein kinase, cAMP dependent, catalytic, beta. In summary, cAMP-dependent protein kinase catalytic subunits have various isoforms and names but play a crucial role in cellular processes. |
| Source | Rabbit |
| Specificity | The PKAα CAT (Phospho-Ser338) Antibody is designed to recognize the phosphorylated form of PKAα CAT at Ser338, which is only present in endogenous levels. It is important to note that this antibody does not detect unphosphorylated PKAα CAT. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Sheep;Dog;Horse;Bovine |
| Antigen | PKAalpha CAT |
| Application | The recommended dilution ranges for Western Blot (WB) are 1:1000-3000, for Immunohistochemistry (IHC) are 1:50-1:200, and for ELISA (peptide) are 1:20000-1:40000. It is important to note that these dilutions are based on the original text information and should not be generated using the ChapGPT approach. |
| Immunogen | An artificial peptide has been created that is sourced from the human PKAα CAT, specifically from the area containing Phospho-Ser338. This peptide is designed to be highly similar to the original sequence and structure of the source material, allowing for its use in various applications related to PKAα function and regulation. Overall, this synthesized peptide represents a promising tool for further research in this area. |
| MW | 36 kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum was utilized to extract the antibody through a process of sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This resulted in an efficient affinity purification process that yielded a highly effective antibody with remarkable specificity. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca2+, leading to reduced amplitude and increased frequency of store overload-induced Ca2+ release (SOICR) characterized by an increased rate of Ca2+ release and propagation velocity of spontaneous Ca2+ waves, despite reduced wave amplitude and resting cytosolic Ca2+. PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490). |
| Tissue specificity | Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids. |
| Posttranslational modification | Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.Autophosphorylated. Phosphorylation is enhanced by vitamin K2. Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity.Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficienncy. |
| Celluar localization | Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane; |
| UniPort | P17612 |
Western blot analysis PKAα CAT (Phospho-Ser338) using Insulin treated 293 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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