Phospho-NF- KappaB P105 (Ser903) Rabbit Polyclonal Antibody#abs139842
Please note that the price mentioned above is provided for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis NF-κB p105 (Phospho-Ser903) using Jurkat whole cell lysates This product is for research use only, not for use in...
Description
| Catalog-specification | Delivery time | USD price |
abs139842-100ug | 1-2 Weeks | 301.0 |
abs139842-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is provided for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent.
| Overview | |
catalog | abs139842 |
| Other names | The DNA binding factor KBF1, also known as EBP1 or NF kappa B, is a transcription factor that plays a key role in the regulation of gene expression in various cellular processes such as inflammation and immune responses. It is encoded by the NFKB1 gene and is composed of two subunits, p50 and p105. KBF1 binds to DNA and activates or represses target genes through interaction with other proteins such as members of the I kappa B family. KBF1 is also known to play a crucial role in the development of various diseases such as cancer, diabetes, and viral infections. Its dysregulation can lead to abnormal gene expression and contribute to disease pathogenesis. Therefore, understanding the molecular mechanisms underlying its function and regulation may provide novel therapeutic targets for the treatment of these diseases. In summary, KBF1/Ebp1/NF kappa B is a versatile transcription factor that plays a critical role in the regulation of gene expression in a variety of cellular processes. Its dysregulation can lead to various diseases, highlighting its importance as a potential therapeutic target. |
| Source | Rabbit |
| Specificity | The NF-κB p105 (Phospho-Ser903) Antibody is specifically designed to detect the presence of phosphorylated NF-κB p105 at Ser903. This antibody is capable of distinguishing only the endogenous levels of the protein when it is phosphorylated at Ser903. |
| Species Reactivity | Human |
| Antigen | NF- kappaB p105 |
| Application | ，：ELISA(peptide)1:20000-1:40000，WB1:1000-3000，IF/ICC1:100-1:500。，ChapGPT。 |
| Immunogen | NF-κB p105（903）...，。 |
| MW | 110kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum is the source of the antibody, which undergoes affinity purification using sequential chromatography techniques with phospho- and non-phospho-peptide columns. This results in a highly purified and specific antibody for its target. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. |
| Tissue specificity | By phorbol ester and TNF. |
| Posttranslational modification | While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.Polyubiquitination seems to allow p105 processing.S-nitrosylation of Cys-61 affects DNA binding.The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. |
| Celluar localization | Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus; |
| UniPort | P19838 |
Western blot analysis NF-κB p105 (Phospho-Ser903) using Jurkat whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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