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Rabbit Anti-alpha Tubulin 4A Polyclonal Antibody#abs133240

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Description

Catalog-specification	Delivery time	USD price
abs133240-100ug	1-2 Weeks	301.0
abs133240-50ug	1-2 Weeks	201.0

Overview
catalog	abs133240
Other names	Alpha tubulin is a highly ubiquitous protein found in various organisms. It is encoded by the TUBA1B gene and is commonly referred to as Tubulin alpha 1B or Tubulin alpha ubiquitous. This protein plays a crucial role in maintaining the structural integrity of cells and is a key component of microtubules. The Tubulin alpha 1B chain is involved in various cellular processes, such as cell division, intracellular transport, and maintenance of cell shape. It is essential for the proper functioning of the cytoskeleton, which provides support and organization to the cell. The Tubulin alpha-ubiquitous chain is widely expressed throughout the body and its expression is not limited to specific cell types or tissues. This ubiquity indicates the importance of this protein in fundamental cellular processes. K-alpha 1 or Tubulin K-alpha-1 refers to the same protein and is another name for Tubulin alpha 1B. This protein is highly conserved across different species, highlighting its essential role in cellular function. In summary, Alpha tubulin, also known as Tubulin alpha 1B or Tubulin alpha ubiquitous, is a vital protein involved in various cellular processes. Its ubiquity and conservation emphasize its significance in maintaining cell structure and function.
Source	Rabbit
Specificity	The antibody against alpha Tubulin 4A is capable of detecting the complete levels of endogenous alpha Tubulin 4A present in the system. This information suggests that the antibody is highly specific to its target protein and can be utilized for various research applications related to alpha Tubulin 4A.
Species Reactivity	Human;Mouse;Rat
Antigen	alpha Tubulin 4A
Application	For western blot, the recommended dilution range is between 1:1000 to 1:3000. When it comes to immunohistochemistry, a dilution of 1:200 is suggested. For both immunocytochemistry and immunofluorescence, there is no specific dilution range mentioned. As for ELISA using peptide, the suggested dilution range is between 1:20000 to 1:40000.
Immunogen	A peptide that has been synthesized from human alpha Tubulin 4A is available. To produce a content that is highly similar to this, all one has to do is make minor adjustments to the original text. The resulting content will be based on the information provided in the original text, but may have a slightly different structure or wording.
MW	50kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the substance, it is recommended to keep it frozen at a temperature of -20 °C for a duration of one year. It is crucial to prevent subjecting the substance to multiple cycles of freezing and thawing.
Storage buffer	The Rabbit IgG is provided in a solution containing phosphate buffered saline with a pH level of 7.4, as well as 150 millimolars of NaCl, 0.02% sodium azide, and 50% glycerol. Storage must be at a temperature of -20 °C, and it is expected to remain stable for a period of 12 months from the date it is received.
Target
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Posttranslational modification	Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution.Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).
Celluar localization	Cytoskeleton;Extracellular region or secreted;
UniPort	P68363