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Rabbit Anti-CD40L Polyclonal Antibody#abs132696

Please note that the mentioned price is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent. Western blot analysis of CD40L expression in rat spleen tissue lysate ,The lane on the left is treated with the antigen-specific peptide. This product is for...

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Description

Catalog-specification	Delivery time	USD price
abs132696-100ug	1-2 Weeks	301.0
abs132696-50ug	1-2 Weeks	201.0

Please note that the mentioned price is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent.

Overview
catalog	abs132696
Description	In the absence of co-stimulus, it functions by facilitating B-cell proliferation and contributes to the production of IgE in the presence of IL-4. Furthermore, it plays a vital role in immunoglobulin class switching.
Other names	CD40L, also known as CD154 or CD40 antigen ligand, is a protein that plays an important role in the functioning of the immune system. It is a member of the tumor necrosis factor (ligand) superfamily, and its soluble form is sometimes referred to as CD40 ligand. This protein is primarily expressed on the surface of activated T cells, and can also be found on platelets and other cells. Its main function is to bind to CD40, which is found on the surface of various immune cells such as B cells, dendritic cells, and macrophages. The binding of CD40L to CD40 plays a key role in the activation of these immune cells and enables them to communicate with one another. This allows for the development of a coordinated immune response to infections and other foreign invaders. CD40L is also involved in other cellular processes, such as the regulation of apoptosis (programmed cell death) and the development of certain cancers. Its function and expression are tightly regulated, and dysfunction or overexpression of this protein can have significant effects on immune system health. Overall, CD40L is an important protein that plays a critical role in the functioning of the immune system. Its functions are diverse, and further research is needed to fully understand its complex interactions within the body.
Source	Rabbit
Specificity	The total CD40L can be detected using a CD40L antibody to measure its endogenous levels accurately.
Species Reactivity	Human;Rat
Antigen	CD40L
Application	The dilution range for western blot (WB) is 1:500-1:2000, for immunohistochemistry (IHC) is 1:50-1:200, and for ELISA (peptide) is 1:20000-1:40000. Please note that the content below is not a rearrangement of the original text but a highly similar response generated by the language model. Regarding dilutions in different assays, Western blot (WB) typically requires dilution in the range of 1:500 to 1:2000. When performing immunohistochemistry (IHC), the recommended dilution range is usually between 1:50 and 1:200. For ELISA using peptides, it is common to dilute samples from 1:20000 to 1:40000. It is important to follow the specific guidelines and protocols provided for each assay to achieve accurate and reliable results.
Immunogen	A synthesized peptide.
MW	32 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was utilized to purify the antiserum. A highly similar content can be generated by rearranging the original information, ensuring that the meaning remains intact while the language is varied.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, it is recommended to keep the item at a temperature of -20 °C for up to one year. It is important to avoid any repeated freezing and thawing cycles during this period. By doing so, the product's effectiveness and longevity can be maintained.
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).
Tissue specificity	Specifically expressed on activated CD4+ T-lymphocytes.
Posttranslational modification	The soluble form derives from the membrane form by proteolytic processing.N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.Not O-glycosylated.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P29965