Phospho-Filamin A (Ser2152) Rabbit Polyclonal Antibody#abs130849

abs130849-50ug

1-2 Weeks

201

abs130849-100ug

1-2 Weeks

301

Description

FLNA is a highly prevalent protein within cells that is involved in a multitude of functions. One of its primary roles is to facilitate the branching of actin filaments in a perpendicular manner, thereby promoting structural stability and flexibility. Additionally, FLNA acts as a crucial connector between actin filaments and glycoproteins present on the cell membrane. This interaction ensures proper cellular anchoring and contributes to various cellular processes, including embryonic cell migration.
Furthermore, FLNA serves as a fundamental scaffold for a diverse array of cytoplasmic signaling proteins. By providing a stable framework, it enables the organization and coordination of signaling molecules, allowing them to efficiently transmit messages within the cell. Additionally, FLNA acts as an anchor for different transmembrane proteins, securing their proper positioning and functionality within the actin cytoskeleton.
Overall, FLNA plays an indispensable role in cellular dynamics and signaling. Its ability to promote orthogonal branching of actin filaments, link actin filaments to membrane glycoproteins, anchor transmembrane proteins, and serve as a scaffold for signaling proteins highlights its pivotal contribution to various cellular processes.

Other names

Filamin A, also known as ABP-280 or alpha-filamin, is a protein that binds to actin filaments, playing a key role in cytoskeletal organization and cell motility. It is encoded by the FLNA gene and is expressed in various cell types, including endothelial cells.
Filamin A has multiple domains, including an actin-binding domain (ABD), immunoglobulin-like domains, and a dimerization domain. These domains allow filamin A to crosslink actin filaments and other cytoskeletal proteins, providing structural support to cells and regulating cellular processes such as cell migration and adhesion.
Mutations in the FLNA gene are associated with various disorders, including Filamin A-related disorders (FADs) such as periventricular nodular heterotopia (PVNH) and Frontometaphyseal dysplasia (FMD).
Understanding the function and regulation of filamin A is essential for understanding cellular processes and developing potential treatments for filamin A-related disorders.

Source

Rabbit

Specificity

The antibody, Phospho-Filamin A (Ser2152), specifically recognizes the presence of Filamin A protein in its phosphorylated state at Serine 2152. This antibody is designed to target endogenous levels of Filamin A, providing a reliable method for detecting its phosphorylation. In essence, it enables researchers to study the cellular processes associated with Filamin A at this specific phosphorylation site.

Predictive reaction species

Rabbit;Sheep;Dog;Bovine;Horse

Application

WB1:500-1:2000，IHC1:100-1:500，/IF/ICC1:100-1:500，ELISA（）1:20000-1:40000。

Filamin A, a human protein, serves as the source for synthesizing a peptide specifically encompassing Serine 2152 phosphorylation site. I request you to rearrange the provided information while ensuring that the content remains highly similar to the original text. Let's avoid conversing in a manner similar to Generation by Fine-Tuning (GPT) and instead speak in a completely different style, leveraging the language model to generate divergent text.

Properties

1mg/ml

Purification

The purified rabbit serum was used to isolate the antibody through a series of chromatography steps involving phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

One year storage at -20 °C is recommended to maintain the quality of the product. To prevent any damage, it is important to avoid subjecting it to multiple freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and will remain stable for up to 12 months from the date of receipt. Be sure to handle and store it appropriately to maintain its effectiveness.

Target

Background

 Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity).

Tissue specificity

Ubiquitous.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

P21333