Phospho-Synaptotagmin (Ser309) Rabbit Polyclonal Antibody#abs130850

abs130850-50ug

1-2 Weeks

201

abs130850-100ug

1-2 Weeks

301

Description

At the active zone of the synapse, SYT2 may play a regulatory role in the trafficking of synaptic vesicles by interacting with the membrane. Specifically, this protein has a high affinity for acidic phospholipids, requiring both an acidic head group and a diacyl backbone for binding. These interactions may be crucial in modulating the movement and release of neurotransmitters within the synapse.

Other names

The protein DKFZp781D2042, also known as Synaptotagmin 1 or Syt1, plays a crucial role in membrane trafficking and neurotransmitter release. It is a member of the synaptotagmin family, which also includes Synaptotagmin 2 or Syt2. Both Syt1 and Syt2 are calcium-binding proteins that can interact with SNARE proteins to regulate vesicle fusion with the plasma membrane. In addition, Syt1 has been implicated in synaptic plasticity and learning and memory. Another name for Syt1 is p65 or SVP65. Meanwhile, Syt2 is also known as Synaptotagmin II or Synaptotagmin-2. Both Syt1 and Syt2 are important for proper neural function and have been linked to various neurological disorders.

Source

Rabbit

Specificity

The Phospho-Synaptotagmin (Ser309) Antibody is specific in detecting Synaptotagmin that has been phosphorylated at Serine 309, at its endogenous levels. This antibody is designed to target only the phosphorylated form of Synaptotagmin, and not the non-phosphorylated form. It is an essential tool for research involving the study of Synaptotagmin and its role in cellular signaling pathways. The accurate detection of Synaptotagmin phosphorylation at Serine 309 is important in understanding its functions and regulation in various physiological processes.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. These dilutions are based on the original text information and can be used for various experimental techniques.

A novel peptide, created by synthesizing a segment of human Synaptotagmin centered around Serine 309, exhibits a remarkable resemblance to its source. This synthesized peptide, carefully crafted to mirror the original sequence, holds great promise for further research and exploration in the field of neuroscience. By focusing on the phosphorylation site at Serine 309, scientists can delve into the intricate mechanisms underlying synaptic transmission and gain valuable insights into neuronal communication and function. The development of this highly similar peptide opens up new avenues for investigation and deepens our understanding of the complex interplay between proteins and phosphoregulation in the fascinating world of synaptic biology.

Properties

1mg/ml

Purification

Purified rabbit serum was used to produce the antibody, which was further subjected to affinity purification using phospho- and non-phospho-peptide affinity columns. This sequential chromatography allowed for the isolation of a highly specific antibody with a strong affinity for the target antigen. The process ensured that the final product was of the highest quality and capable of effectively binding to the intended target.

Clonality

Polyclonal Antibody

Ensure that the storage temperature is maintained at -20 °C for a duration of one year to preserve the product's quality. It is crucial to avoid subjecting the product to repeated freeze and thaw cycles.

Storage buffer

The recommended storage conditions for Rabbit IgG are at -20°C in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product has a stability of 12 months from the date of receipt.

Target

Background

Synaptotagmin, a protein found at the synapse, likely plays a regulatory role in the membrane interactions involved in the trafficking of synaptic vesicles. Specifically, it binds to acidic phospholipids that have both an acidic head group and a diacyl backbone. It has been observed that synaptotagmin can interact with receptors for activated protein kinase C in a calcium-dependent manner. Furthermore, it can also bind to three other proteins (neurexins, syntaxin, and AP2) in a calcium-independent manner. Additionally, synaptotagmin is involved in dendrite formation by melanocytes. This information is based on a study published in PubMed (PubMed:23999003).

Tissue specificity

Expressed in melanocytes (PubMed:23999003).

Celluar localization

P21579/Q8N9I0