Rabbit Anti-TGF Alpha Polyclonal Antibody #abs130198

abs130198-50ug

1-2 Weeks

201

abs130198-100ug

1-2 Weeks

301

Description

TGFalpha is a peptide that can stimulate cell growth and is able to bind to the EGF receptor. In addition, TGFalpha works with TGF beta to promote cell proliferation in a soft agar environment. This unique property has made TGFalpha an attractive target for further research on its potential role in promoting cancer cell growth and metastasis.

Other names

Transforming growth factor alpha, also known as TGF-alpha or ETGF, is a protein involved in cellular growth and development. It is part of the EGF-like TGF family and is closely related to other members such as TGF type 1 and waved 1.
TGF-alpha is produced as a precursor molecule, known as Transforming growth factor alpha precursor, which undergoes specific cleavage to generate the biologically active protein. The protein is involved in the formation and maintenance of tissues in the body and is also known to promote the growth of cancer cells.
TGF-alpha binds to the same receptors as epidermal growth factor (EGF), which are present on the surface of many different types of cells. Upon binding, it triggers the signaling pathways that lead to cell proliferation and differentiation.
TGF-alpha is found in various tissues throughout the body, including the gastrointestinal tract, liver, and brain. In addition to its role in normal physiology, TGF-alpha has been implicated in the pathogenesis of several diseases, including cancer and inflammatory bowel disease.
Overall, the ETGF protein is an important regulator of cellular growth and development, with a wide range of functions throughout the body. Its close relationship to other members of the EGF-like TGF family suggests that it may play a key role in coordinating the activities of these proteins within cells and tissues.

Source

Rabbit

Specificity

The TGF alpha antibody is capable of detecting all forms of TGF alpha present in an organism. It is able to accurately measure the levels of this protein and determine its total concentration within the system. This information is pertinent for many applications, including disease diagnosis and treatment planning. Through its ability to recognize endogenous levels of TGF alpha, this antibody has become an important tool in modern medicine. Its high specificity and sensitivity make it a valuable asset for researchers and medical professionals alike.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Bovine;Horse

Application

For Western Blotting, the recommended dilution range is from 1:500 to 1:3000. When performing Immunohistochemistry, it is suggested to use a dilution range of 1:50 to 1:200 for optimal results. For Immunofluorescence and Immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. When performing ELISA with a peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to follow these suggested dilution ranges for the best results in your experiments.

Immunogen

Synthetic peptides are created by rearranging the amino acids in specific sequences of a protein. In the case of human TGF alpha, a synthesized peptide is derived from this protein. The process involves selecting the amino acids and their order to reproduce a highly similar peptide structure. This allows researchers to investigate the functions and properties of TGF alpha in a controlled and specific manner. The synthesized peptide derived from human TGF alpha can be further studied to gain insights into its role in various biological processes and potential therapeutic applications.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. To generate similar content, the information presented should remain consistent while the wording is rearranged in a way that conveys the same meaning.

Clonality

Polyclonal Antibody

Stability & Storage

The storage condition for one year is -20 °C. It is important to avoid subjecting the stored item to repeated cycles of freezing and thawing.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the product at a temperature of -20 °C for optimal stability, with a shelf life of 12 months from the date of receipt. Please ensure proper handling and storage of the product to maintain its quality and efficacy.

Target

Background

TGF alpha, a polypeptide with mitogenic properties, possesses the capability to bind with the EGF receptor/EGFR. It exhibits synergistic effects when combined with TGF beta, thereby facilitating cell proliferation in soft agar even in the absence of anchorage.

Tissue specificity

Keratinocytes and tumor-derived cell lines express isoform 1, isoform 3 and isoform 4. To rephrase this, we can say that isoform 1, isoform 3 and isoform 4 are present in both keratinocytes and tumor-derived cell lines.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

UniPort

P01135