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Rabbit Anti-NUMBL Polyclonal Antibody#abs136383

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Description

Catalog-specification	Delivery time	USD price
abs136383-100ug	1-2 Weeks	301.0
abs136383-50ug	1-2 Weeks	201.0

Overview
catalog	abs136383
Description	Numbl, also known as Numb-like, plays a pivotal role in the Notch signaling pathway by binding to Notch as an intracellular modulator. This protein features a phosphotyrosine binding domain that can interact with the Y682 motif present in APP's cytoplasmic tail. The predicted molecular weight of Numbl is approximately 72 kDa. By rearranging the information provided in the original text, we can reiterate that Numbl is a significant actor in cell signaling pathways, particularly in the context of Notch and APP interactions.
Other names	Numb homolog-like proteins, also known as NBL or NUMB Drosophila Homolog Like, play a crucial role in various biological processes. These proteins are encoded by genes such as CAG 3A, CTG 3a, NUMBL, and TNRC 23. They share a high degree of similarity with the Numb protein found in Drosophila. Numb homolog-like proteins are involved in cellular signaling pathways and have been implicated in the regulation of cell fate determination, neuronal development, and tumor suppression. Their function is mediated through interactions with other proteins and modulation of intracellular signaling cascades. The NUMBL gene, also known as Numb-related gene, encodes a protein that is highly similar to Numb and exerts similar functions. NUMBL protein is particularly expressed in the nervous system and has been associated with neural stem cell regulation and neuronal differentiation. Another member of the Numb homolog-like proteins, designated as NUMBR or Numb-R, has also been identified. NUMBR shares significant sequence similarity with Numb and NUMBL and exhibits similar subcellular localization and function. It plays a role in diverse cellular processes, including cell polarity, cell migration, and immune response. In summary, Numb homolog-like proteins, including NUMBL and NUMBR, are important regulators of cellular processes and have broad implications in development and disease. Their resemblance to the Drosophila Numb protein highlights their evolutionary conservation and significance in biology.
Source	Rabbit
Specificity	The NUMBL Antibody is specifically designed to recognize and bind to total NUMBL present in the body. This antibody is capable of detecting endogenous levels of total NUMBL, making it a valuable tool for research purposes. By rearranging the original text, this new version conveys the same information in a slightly different way.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;Dog
Antigen	NUMBL
Application	The dilution ranges for WB, IHC, IF/ICC, and ELISA(peptide) are as follows: - WB: 1:500 to 1:2000 - IHC: 1:50 to 1:200 - IF/ICC: 1:100 to 1:500 - ELISA(peptide): 1:20000 to 1:40000 To ensure accurate results, it is crucial to dilute the samples properly within these ranges for each respective technique. WB requires a higher dilution compared to the other methods, with a range of 1:500-1:2000. For IHC, a dilution range of 1:50-1:200 is recommended. IF/ICC should be performed using a dilution range of 1:100-1:500. Finally, when performing ELISA using a peptide, the appropriate dilution range is 1:20000-1:40000. Following these guidelines will help obtain reliable and consistent results in these experimental techniques.
Immunogen	A synthesized peptide derived from human NUMBL.
MW	65kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the peptide affinity chromatography process to purify the antiserum. Let me generate a highly similar content by rearranging the above information while maintaining its original context.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
UniPort	Q9Y6R0