Phospho-MARCKS (Ser158) Rabbit Polyclonal Antibody#abs130877

abs130877-50ug

1-2 Weeks

201

abs130877-100ug

1-2 Weeks

301

Description

Protein kinase C primarily targets MARCKS as its cellular substrate, making it the most prominent one. MARCKS has the ability to bind calmodulin, actin, and synapsin. It functions as a key protein in linking filamentous actin, contributing to the formation of cross-links. This protein plays a critical role in the normal development of the brain. To summarize, MARCKS is essential for brain development and exhibits binding affinity towards calmodulin, actin, and synapsin, while also functioning as a filamentous actin cross-linking protein.

Other names

The 80K L protein is also known as myristoylated alanine rich C kinase substrate (MARCKS). It is an 80 kDa protein and plays a pivotal role in cellular signaling and membrane dynamics. The protein is a substrate for protein kinase C and is involved in the regulation of actin cytoskeleton, neurotransmitter release, and cell migration. The protein can be found in various tissues including brain, heart, lung, and kidney.
In addition to its role as a substrate for protein kinase C, the 80K L protein also interacts with other proteins and lipids in the plasma membrane. It contains a myristoylation site at the N-terminus, which allows for its attachment to the plasma membrane. The protein also contains multiple phosphorylation sites, which regulate its function and localization within the cell.
The 80K L protein has been implicated in various cellular processes including cell migration, synaptic plasticity, and cancer metastasis. Dysregulation of the protein has been linked to several human diseases including Alzheimer's disease, bipolar disorder, and cancer.
Despite its diverse roles in cellular signaling and disease, much remains to be discovered about the 80K L protein. Further research is needed to fully understand its function and potential therapeutic applications.

Source

Rabbit

Specificity

The Phospho-MARCKS (Ser158) Antibody is specifically designed to identify and measure the levels of phosphorylated MARCKS protein at Serine 158 in biological samples. It is not capable of detecting non-phosphorylated MARCKS or phosphorylation at other sites.

Predictive reaction species

Pig;Xenopus;Chicken;Bovine;Rabbit

Application

The recommended dilutions for Western blotting are 1:500 to 1:2000, while for ELISA using peptides, the suggested dilution range is between 1:20000 to 1:40000. To reiterate, the Western blotting protocol should involve diluting the sample to a ratio of 1:500 to 1:2000, while for ELISA with peptides, dilutions should be made in the range of 1:20000 to 1:40000 for optimal results.

A peptide that has been synthesized from human MARCKS has been designed to target the phosphorylation site located at Serine 158. Alterations have been made to the original sequence in order to create a similar but distinct product.

Properties

1mg/ml

Purification

，。，，。

Clonality

Polyclonal Antibody

To preserve the item's quality, it is recommended to store it at a temperature of -20 °C for a year. It is important to prevent it from being subjected to repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG that has been formulated with phosphate buffered saline (pH 7.4), 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20 °C for best results. The product is stable for 12 months from the date of receipt.

Target

Background

 MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Celluar localization

Cytoskeleton;Extracellular region or secreted;Nucleus;Plasma Membrane;

P29966