Phospho-Cyclin D3 (Thr283) Rabbit Polyclonal Antibody#abs130878

abs130878-50ug

1-2 Weeks

201

abs130878-100ug

1-2 Weeks

301

Description

The CCND3 protein is a crucial regulator of the cell cycle during the G(1)/S transition. It acts as a regulatory component of the cyclin D3-CDK4 complex and plays a vital role in phosphorylating and inhibiting members of the retinoblastoma protein family, such as RB1. This activity of the cyclin D3-CDK4 complex is necessary to effectively regulate the transition from the G(1) phase to the S phase of the cell cycle. Through its action, CCND3 ensures that cells only proceed to the next phase of the cell cycle once they have completed all necessary processes, thereby preventing uncontrolled cell proliferation.

Other names

The gene CCND3 is responsible for the production of cyclin D3, a protein that plays an important role in regulating the cell cycle. Cyclin D3 is specifically involved in the G1 to S phase transition, making it a G1/S-specific cyclin. Additionally, Cyclin D3 is also referred to as D3 type cyclin and G1 S specific cyclin D3. The protein encoded by the CCND3 gene is highly similar to the human CCND3_HUMAN gene, suggesting that it may have similar functions in other organisms. Overall, the CCND3 gene and its corresponding protein are crucial components in the regulation of cell division and proliferation.

Source

Rabbit

Specificity

The ability of Phospho-Cyclin D3 (Thr283) Antibody to detect Cyclin D3 is restricted to cases where it is specifically phosphorylated at Threonine 283. It is important to note that this antibody is designed to target endogenous levels of Cyclin D3 in samples. To elaborate, Cyclin D3, when phosphorylated at Thr283, can be reliably detected using this antibody.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The recommended dilution ratios for using this antibody in various applications are as follows: For Western blotting, dilute it at 1:500-1:2000; for immunohistochemistry, dilute it at 1:50-1:200; for immunofluorescence or immunocytochemistry, dilute it at 1:100-1:500; and for ELISA with peptide, dilute it at 1:20000-1:40000. To ensure optimal results, please follow the instructions provided in the protocols for each application. These recommended dilutions have been determined based on the original text information.

A peptide that has been artificially created and is derived from a segment of human Cyclin D3 located in the vicinity of the Threonine 283 phosphorylation site, is referred to as synthesized peptide. It is designed to be highly similar in composition to the original protein sequence.

Properties

1mg/ml

Purification

The antibody was obtained through a rigorous purification process involving sequential chromatography on both phospho- and non-phospho-peptide affinity columns. The source of the antibody was rabbit serum which was purified using affinity methods to ensure high purity and specificity.

Clonality

Polyclonal Antibody

To maintain the potency of the product, it is advised to store it at a temperature of minus 20 degrees Celsius for a period of one year. It is important to note that exposing the product to frequent changes in temperature due to freeze and thaw cycles could adversely impact its effectiveness. Hence, it is recommended to avoid such temperature fluctuations as much as possible.

Storage buffer

This product consists of Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store at -20 °C and it is stable for 12 months from the date of receipt.

Target

Background

The cyclin D3-CDK4 (DC) complex acts as a regulatory component that performs phosphorylation and inhibition on members of the retinoblastoma (RB) protein family, such as RB1. Its primary role is to regulate the cell-cycle progression specifically during the G1/S transition. By phosphorylating RB1, this complex facilitates the dissociation of the transcription factor E2F from the RB/E2F complex. Consequently, E2F target genes become available for transcription, leading to the necessary progression through the G1 phase. During early G1 phase, RB1 is hypophosphorylated by the cyclin D3-CDK4 complex. This complex serves as a major integrator, responding to both mitogenic and antimitogenic signals. In addition, it acts as a substrate for SMAD3, undergoing phosphorylation in a cell-cycle-dependent manner. This phosphorylation event is responsible for repressing the transcriptional activity of SMAD3. Furthermore, the cyclin D3-CDK4 complex is a vital component of the ternary complex, cyclin D3/CDK4/CDKN1B, which is necessary for both the nuclear translocation and activity of the cyclin D-CDK4 complex.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P30281