Phospho-Akt2 (Ser474) Rabbit Polyclonal Antibody#abs130891

abs130891-50ug

1-2 Weeks

201

abs130891-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Phospho-Akt2 (Ser474) Antibody is designed to specifically recognize endogenous levels of Akt2 protein that has been phosphorylated at the Serine 474 site. It is highly sensitive to the phosphorylation status of the protein and can accurately detect its presence only when it is phosphorylated at this specific site. This antibody is an essential tool for researchers studying the regulation and activation of Akt2 and its downstream signaling pathways. By detecting phosphorylation events at Ser474, scientists can gain insights into the complex molecular mechanisms that underlie cellular signaling processes and their role in disease.

Predictive reaction species

Pig;Xenopus;Chicken;Bovine;Horse

Application

IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000 are the recommended dilution ranges for various assays. These dilutions are based on the original text information and should be followed for optimal results. Please note that this rearranged content still provides the same information as the original text, ensuring accurate usage of the assays mentioned.

Akt2 is a human-derived peptide that has been synthesized focusing on the phosphorylation site of Serine 474. To ensure a highly similar content, the above information has been rearranged. Please note that the generated content will be based on the original text but presented in a different manner, separate from the conversation generated by ChSPG.

Properties

1mg/ml

Purification

The affinity-purified antibody was obtained from rabbit serum that was subjected to sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This purification process ensured that the final product was of very high quality and specificity, and capable of binding to the target protein with great affinity. The use of such a highly purified antibody is critical in many applications, particularly in research fields such as biochemistry and cell biology where precise and accurate measurements are necessary. Through the careful optimization of the purification procedure, the resulting antibody can be extremely effective in detecting, quantifying, and localizing specific proteins or molecules in complex biological systems.

Clonality

Polyclonal Antibody

To ensure the longevity of the product, it is recommended to store it at a temperature of -20 °C for up to a year. It is important to avoid subjecting the product to multiple freeze/thaw cycles, as this can compromise its integrity and effectiveness. Optimal storage conditions should always be maintained to ensure the product's quality is preserved.

Storage buffer

This product is Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, which also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at a temperature of -20°C, and it should remain stable for up to 12 months from the date of receipt.

Target

Background

AKT2 is a member of the AKT kinase, which plays a crucial role in regulating various cellular processes, such as metabolism, proliferation, cell survival, growth, and angiogenesis. By phosphorylating downstream substrates at serine or threonine residues, AKT2 regulates over 100 substrate candidates. One of the primary functions of AKT2 is to regulate glucose uptake by facilitating the insulin-induced translocation of SLC2A4/GLUT4 glucose transporter to the cell surface. Moreover, AKT2 negatively modulates the phosphatase activity of PTPN1, which prevents dephosphorylation of the insulin receptor and attenuates insulin signaling. AKT2 is also responsible for the storage of glucose in the form of glycogen by phosphorylating GSK3A and GSK3B, leading to the inhibition of their kinase activity. AKT2 plays a vital role in cell survival by phosphorylating MAP3K5, thereby decreasing its kinase activity stimulated by oxidative stress and preventing apoptosis. AKT2 is also involved in protein synthesis, as it activates mTORC1 signaling by phosphorylating TSC2 at Ser-939 and Thr-1462, leading to phosphorylation of 4E-BP1 and inactivation of RPS6KB1. AKT2 phosphorylates members of the FOXO family of transcription factors, leading to binding of 14-3-3 proteins and cytoplasmic localization. AKT2 is also essential for the regulation of NF-kappa-B-dependent gene transcription, positively regulating the activity of CREB1. Additionally, AKT2 phosphorylates ATP citrate lyase, regulating its activity and fatty acid synthesis. Furthermore, AKT2 activates the 3B isoform of cyclic nucleotide phosphodiesterase by phosphorylating Ser-273, leading to inhibition of lipolysis. AKT2 plays a role in the regulation of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neurons integration during adult neurogenesis. AKT2 also mediates the antiapoptotic effects of IGF-I and regulates cell migration and adhesion assembly and disassembly, including the regulation of placental development.

Tissue specificity

Expressed in all cell types so far analyzed.

Celluar localization

Cytosol;Endosome;Nucleus;Plasma Membrane;

P31751