Rabbit Anti-GRP78 Polyclonal Antibody #abs130538

abs130538-50ug

1-2 Weeks

201

abs130538-100ug

1-2 Weeks

301

Description

GRP78 is a crucial member of the HSP family, known as molecular chaperones, which are essential for maintaining the integrity of the endoplasmic reticulum and facilitating stress-induced autophagy. Its role in regulating the unfolded protein response (UPR) is pivotal, and it is also an obligatory component for autophagy in mammalian cells. Notably, GRP78 may hold significance in cellular adaptation and the survival of oncogenic cells. An interesting connection lies between GRP78 and the protein called double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK), where GRP78 acts as a chaperone for PERK. This interaction underscores the importance of GRP78 in cellular functions and highlights the intricacies of protein folding and response to stress within the endoplasmic reticulum.

Other names

The 78 kDa glucose regulated protein, also known as GRP78 or BiP, is a Ca(2+)-binding protein located in the endoplasmic reticulum lumen. This protein is encoded by multiple genes including AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, and MIF2. It is also referred to as the heat shock protein family A (Hsp70) member 5 or heat shock 70kDa protein 5. Other names for GRP78 include the immunoglobulin heavy chain-binding protein and epididymis secretory sperm binding protein Li 89n.
GRP78 is a key regulator of protein folding and secretion in the endoplasmic reticulum. It plays a central role in the protection of cells from stress, including heat shock, hypoxia, and nutrient deprivation. GRP78 is highly expressed in a variety of tissues, including the brain, heart, liver, and pancreas. It has been associated with various pathologies, including cancer, diabetes, and neurodegenerative diseases.
The discovery of GRP78 as a critical regulator of cellular homeostasis has opened up new avenues for research and potential therapeutic interventions. Further elucidation of its functions and interactions with other proteins and pathways may lead to the development of novel treatments for various diseases.

Source

Rabbit

Specificity

GRP78 Antibody detects endogenous levels of GRP78.

Species Reactivity

Human;Mouse;Rat;Monkey

Application

The recommended dilutions for WB are between 1:500 and 1:2000, while for IHC the range is between 1:50 and 1:200. As for IF/ICC, the suggested dilutions are between 1:100 and 1:500. ELISA tests using peptide usually require a dilution of 1:20000 to 1:40000. It is important to keep in mind these recommended dilutions to ensure accurate results in your experiments.

Immunogen

A synthesized peptide derived from human GRP78.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to perform peptide affinity chromatography for the purification of the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

One year of storage is recommended at -20 °C. Prevent repeated freezing and thawing cycles to maintain the quality of the stored items.

Storage buffer

The storage condition for Rabbit IgG is in phosphate buffered saline at pH 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is stable for a period of 12 months from the date of receipt. Please note that the content generated by rearranging the information should be based on the original text and should not follow the conversation style of ChapGPT.

Target

Background

The assembly of multimeric protein complexes within the endoplasmic reticulum is aided by this protein's function. It plays a key role in the correct folding of proteins and contributes to the degradation of misfolded proteins by interacting with DNAJC10, which likely aids in the detachment of DNAJC10 from its substrate.

Tissue specificity

By endoplasmic reticulum stress.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;

UniPort

P11021