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Rabbit Anti-BUB1B Polyclonal Antibody#abs133036

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Description

Catalog-specification	Delivery time	USD price
abs133036-50ug	1-2 Weeks	201.0
abs133036-100ug	1-2 Weeks	301.0

Overview
catalog	abs133036
Description	The mitotic checkpoint is an essential component for the proper progression of mitosis. Its primary role is to delay the onset of anaphase, the separation of sister chromatids, until all chromosomes have successfully attached to the mitotic spindle. To achieve this, the checkpoint inhibits the activity of the anaphase-promoting complex/cyclosome (APC/C) by preventing the binding of CDC20, an activator protein, to the complex. Importantly, this inhibition occurs independently of the checkpoint's kinase activity. Another critical function of the mitotic checkpoint is to monitor the activities of kinetochores, specialized protein structures that attach chromosomes to the mitotic spindle. Specifically, it is involved in ensuring the appropriate localization of CENPE, a motor protein essential for kinetochore function. Furthermore, the mitotic checkpoint acts as a negative regulator of PLK1, a key protein involved in cell division, during interphase. It also suppresses the amplification of centrosomes, structures that aid in chromosome segregation. In addition to its pivotal role in mitosis, the mitotic checkpoint has been implicated in triggering apoptosis, programmed cell death, in polyploid cells that incorrectly exit from a state of mitotic arrest. This function highlights its involvement in maintaining genomic stability. Overall, the mitotic checkpoint plays a crucial role in normal mitosis progression, ensuring accurate chromosome segregation and preventing the formation of abnormal cells. Its functions extend beyond mitosis and may contribute to tumor suppression, emphasizing its importance in maintaining cellular integrity.
Other names	S. cerevisiae BUB1 is a known budding uninhibited by benzimidazoles homolog, specifically the beta homolog. It is also referred to as BUB1B, BUB1B_HUMAN, Bub1A, BUB1beta, BUBR1, and MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 beta. Another name for this protein is Budding uninhibited by benzimidazoles 1 homolog beta (yeast) or Mitotic checkpoint kinase MAD3L. It is encoded by the MAD3L gene and is a member of the MAD3 protein kinase family. In addition, this protein is associated with the regulation of the mitotic checkpoint and plays a crucial role in cell division and genomic stability. Its function involves monitoring chromosome alignment and spindle attachment during mitosis. The SSK1 protein, also known as Protein SSK1, is closely related to BUB1B and is considered a beta homolog. The identification and understanding of these proteins are significant in studying cell cycle regulation and the mechanisms that maintain genomic integrity. By rearranging the provided information, we can emphasize the various names and functions associated with S. cerevisiae BUB1 and its homologs.
Source	Rabbit
Specificity	Total BUB1B levels can be detected by the BUB1B antibody, effectively identifying endogenous expression. A rearranged version of the content: The BUB1B antibody is capable of detecting total BUB1B levels, thus allowing for the identification of endogenous expression.
Species Reactivity	Human;Mouse;Rat
Antigen	BUB1B
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	119 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Tissue specificity	Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
Posttranslational modification	Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.Ubiquitinated. Degraded by the proteasome.Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	O60566